Description

Cyclooctyne-o-Nhs Ester is a highly reactive, bifunctional chemical linker designed for bioorthogonal conjugation strategies. This compound matters because it enables efficient, catalyst-free labeling of biomolecules under physiological conditions, a critical requirement for modern life science research and diagnostics. Researchers and manufacturers in the fields of bioconjugation, chemical biology, and diagnostic probe development need this reagent for its ability to facilitate stable amide bond formation via strain-promoted azide-alkyne cycloaddition (SPAAC) and subsequent NHS ester chemistry.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of antibodies, enzymes, and other proteins for creating diagnostic and therapeutic conjugates.
• Chemical Biology Probes: Synthesis of activity-based probes (ABPs) and imaging agents for studying biological processes in live cells.
• Diagnostic Assay Development: Conjugation of detection molecules (e.g., fluorophores, enzymes) to targeting ligands in lateral flow assays and ELISA.
• Polymer and Material Science: Functionalization of surfaces, nanoparticles, and hydrogels with bioactive ligands for tissue engineering and drug delivery systems.
• Next-Generation Sequencing (NGS): Preparation of labeled nucleotides or oligonucleotides for sequencing applications.
• ADC (Antibody-Drug Conjugate) Research: As a tool compound for exploring novel linker-payload attachment strategies in oncology research.

Basic Information

Product Name	Cyclooctyne-o-Nhs Ester
CAS No.	1425803-45-7
Molecular Formula	C₁₈H₂₀N₂O₆
Molecular Weight	360.36 g/mol
Synonyms	Cyclooctyne NHS Ester; 1-(4-((1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethoxy)-4-oxobutanoyloxy)pyrrolidine-2,5-dione; DBCO-PEG4-NHS Ester; Dibenzocyclooctyne-PEG4-NHS Ester; DBCO-PEG4-NHS; DBCO-SE-PEG4; Azadibenzocyclooctyne-PEG4-N-hydroxysuccinimide ester; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl 4-((2,5-dioxopyrrolidin-1-yl)oxy)-4-oxobutanoate
EINECS	Contact for details

Quality Control

Our Cyclooctyne-o-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and MS, to ensure it meets the high standards required for sensitive research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.