Description

Endo-Bcn-Peg4-Pfp Ester is a high-purity, heterobifunctional crosslinking reagent featuring an endo-BCN group and a pentafluorophenyl (PFP) ester, connected by a tetraethylene glycol (PEG4) spacer. This compound is engineered for efficient, selective bioconjugation, particularly in demanding fields like antibody-drug conjugate (ADC) development and proteomics research. It is an essential tool for researchers and manufacturers in pharmaceutical R&D, biotech, and advanced materials science who require reliable, site-specific labeling and stable linker chemistry. The product is supplied under stringent quality control to ensure optimal performance and batch-to-batch consistency.

Application

• Antibody-Drug Conjugate (ADC) Payload/Linker Conjugation: The PFP ester reacts efficiently with amine groups on antibodies or proteins, while the endo-BCN group undergoes rapid, bioorthogonal strain-promoted alkyne-azide cycloaddition (SPAAC) with azide-functionalized cytotoxic payloads.
• Protein and Peptide Labeling/Modification: Used for site-specific introduction of the BCN moiety onto biomolecules for subsequent click chemistry with azide-tagged probes, fluorophores, or other functional groups.
• Surface Functionalization and Biomaterial Engineering: Enables the covalent attachment of bioactive molecules to azide-presenting surfaces, hydrogels, or nanoparticles for cell culture, biosensing, and tissue engineering applications.
• Chemical Biology and Proteomics Tool Development: Serves as a key reagent for constructing activity-based probes (ABPs) or affinity tags to study enzyme function, protein-protein interactions, and cellular pathways.
• Polymer and Dendrimer Synthesis: Acts as a versatile building block for creating functionalized polymers with precise, clickable handles for further modular assembly.
• Diagnostic Reagent and Kit Manufacturing: Incorporated into the development of sensitive immunoassays and diagnostic kits that utilize click chemistry for signal amplification or detection.

Basic Information

Product Name	Endo-Bcn-Peg4-Pfp Ester
CAS No.	1421932-52-6
Molecular Formula	C₃₀H₃₄F₅NO₉
Molecular Weight	623.59 g/mol
Synonyms	endo-BCN-PEG4-PFP ester; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl (1-oxo-2,5,8,11-tetraoxatetradecan-14-yl) (2,3,4,5,6-pentafluorophenyl) carbonate; 2,5,8,11-Tetraoxatetradecan-14-oic acid, 14-[[(1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-yl]methyl] 2,3,4,5,6-pentafluorophenyl ester; endo-BCN-PEG4-PFP; BCN-PEG4-PFP Ester; BCN-PEG4-PFP; PFP-PEG4-endo-BCN
EINECS	Contact for details

Quality Control

Our Endo-Bcn-Peg4-Pfp Ester is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure it meets the high-purity standards required for sensitive bioconjugation applications. Each batch is characterized by NMR, HPLC, and mass spectrometry to confirm identity, purity, and absence of critical impurities. We adhere to a strict quality management system, and a comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or oil
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms (M+Na⁺ or M+H⁺)
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.