Description

5-FITC-PEG3-Azide (CAS NO 1402745-37-2) is a high-purity, trifunctional fluorescent labeling reagent designed for advanced bioconjugation and chemical biology research. This compound integrates a fluorescein isothiocyanate (FITC) fluorophore with a triethylene glycol (PEG3) spacer and a terminal azide group, enabling precise, click chemistry-based tagging of biomolecules. It is essential for researchers and developers in pharmaceutical R&D, diagnostic assay development, and proteomics who require reliable, site-specific fluorescent probes for imaging, detection, and biomolecular tracking applications.

Application

• Bioconjugation & Probe Development: Site-specific labeling of proteins, antibodies, and oligonucleotides via copper-free or copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry.
• Fluorescence Imaging & Microscopy: Preparation of fluorescent tags for cellular imaging, flow cytometry (FACS), and in vivo tracking studies due to the bright green emission of the FITC moiety.
• Diagnostic Assay Reagent: Used in the development of fluorescent immunoassays (FLISA), lateral flow tests, and other biosensing platforms.
• Chemical Biology & Proteomics: A key tool for activity-based protein profiling (ABPP), target identification, and studying biomolecular interactions.
• Polymer & Material Science Functionalization: Introducing fluorescent and click-reactive handles onto surfaces, nanoparticles, hydrogels, and other polymeric materials for traceability and further modification.
• Pharmaceutical Research: Labeling of drug candidates, lipids, or nanoparticles to study their pharmacokinetics, biodistribution, and cellular uptake mechanisms.

Basic Information

Product Name	5-FITC-PEG3-Azide
CAS No.	1402745-37-2
Molecular Formula	C33H35N5O9S
Molecular Weight	677.73 g/mol
Synonyms	5-Fluorescein-PEG3-Azide; FITC-PEG3-N3; 5-Isothiocyanatofluorescein-PEG3-Azide; Fluorescein-5-isothiocyanate triethylene glycol azide; 6-(2-(2-(2-Azidoethoxy)ethoxy)ethoxy)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-yl carbonothioic O-ester; Azido-PEG3-FITC; FITC-PEG3-N3
EINECS	Contact for details

Quality Control

Our 5-FITC-PEG3-Azide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and structural confirmation via NMR and mass spectrometry. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and impurity profiles to ensure compliance with research and development standards. Custom synthesis and specific purity grades (e.g., >95%, >98%) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed bag with silica gel) due to its hygroscopic and strictly light-sensitive nature. For long-term storage, keep under an inert atmosphere (e.g., argon or nitrogen) to prevent degradation. Allow the product to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.