Description

Dbco-Sulfo-Nhs Ester is a water-soluble, amine-reactive chemical crosslinker designed for efficient bioconjugation. This compound is critical for creating stable, site-specific linkages between biomolecules and various probes or surfaces under mild, aqueous conditions. It is primarily needed by researchers and manufacturers in the fields of biopharmaceutical development, diagnostic assay production, and advanced materials science for applications like antibody-drug conjugate (ADC) synthesis and surface functionalization.

Application

• Antibody-Drug Conjugate (ADC) Synthesis: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies for targeted cancer therapies.
• Diagnostic Assay Development: Immobilization of proteins, peptides, or oligonucleotides onto sensor chips, microplates, or nanoparticle surfaces for ELISA, lateral flow assays, and biosensors.
• Biomolecule Labeling: Covalent attachment of fluorescent dyes, biotin, or other reporter molecules to proteins, antibodies, or nucleic acids for detection and imaging.
• Polymer and Material Functionalization: Modification of hydrogels, dendrimers, or other polymeric materials with biological ligands to create bioactive scaffolds for tissue engineering or drug delivery.
• Click Chemistry Applications: Utilizes strain-promoted azide-alkyne cycloaddition (SPAAC) for rapid, catalyst-free conjugation with azide-modified molecules, ideal for live-cell labeling.
• Protein-Protein Crosslinking: Studying protein interactions or creating protein complexes for structural biology and therapeutic applications.

Basic Information

Product Name	Dbco-Sulfo-Nhs Ester
CAS No.	1400191-52-7
Molecular Formula	C26H23N3O9S
Molecular Weight	553.54 g/mol
Synonyms	DBCO-sulfo-NHS ester; Dibenzocyclooctyne-sulfo-N-hydroxysuccinimide ester; Sulfo-DBCO-NHS ester; DBCO-PEG4-sulfo-NHS ester; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-(2,5-dioxo-1-sulfo-2,5-dihydro-1H-pyrrol-3-yl)carbamate; Azadibenzocyclooctyne-sulfo-NHS; SULFO-SANPAH analog (DBCO-based); Amine-reactive DBCO crosslinker
EINECS	Contact for details

Quality Control

Our Dbco-Sulfo-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive bioconjugation work. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 90% (by UV-Vis)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.