Description

Biotinyl Pyridinyldithio n-Hydroxysuccinimide Alanine Ester is a specialized heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is critical for researchers and manufacturers who require precise, reversible linkage between biomolecules, such as attaching biotinylated probes to thiol-containing proteins or peptides. It is primarily utilized in the development of diagnostic assays, targeted drug delivery systems, and advanced biochemical research tools where controlled and cleavable conjugation is essential.

Application

• Bioconjugation Reagent: For the reversible, site-specific crosslinking of biotinylated molecules (e.g., antibodies, nucleic acids) to thiol-containing targets.
• Diagnostic Assay Development: Used in the fabrication of ELISA, lateral flow assays, and other immunoassays that require cleavable detection systems.
• Drug Delivery & Targeting: Enables the construction of antibody-drug conjugates (ADCs) or other targeted therapeutics with a disulfide linker for controlled release.
• Affinity Chromatography: Immobilization ligand for preparing reversible affinity columns to purify thiol-containing proteins.
• Surface Functionalization: Modification of gold nanoparticles, biosensors, or chips with biotin for subsequent streptavidin-based assembly.
• Protein & Peptide Labeling: Introduction of a biotin tag onto cysteine residues for detection, pull-down assays, or imaging.
• Biochemical Research: A key tool in studying protein-protein interactions, cellular trafficking, and signal transduction pathways.

Basic Information

Product Name	Biotinyl Pyridinyldithio n-Hydroxysuccinimide Alanine Ester
CAS No.	1356933-91-9
Molecular Formula	C23H31N5O6S2
Molecular Weight	545.65 g/mol
Synonyms	Biotin-PDPH-Ala-OSu; Biotin-PDPH-Alanine-NHS Ester; Biotin-PDPH-Ala-ONSu; N-(Biotinyl)-S-(2-pyridinyldithio)alanine N-hydroxysuccinimide ester; Biotin-PDPH-Ala-OSuc; Biotin-PDPH-Ala-NHS; Biotin-PDPH-Ala-SE; (2S)-2-[(5-{3-[(2S)-2-[(2,5-Dioxopyrrolidin-1-yl)oxy]-2-oxoethyl]disulfanyl}pyridin-2-yl)disulfanyl]-5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid
EINECS	Contact for details

Quality Control

Our Biotinyl Pyridinyldithio n-Hydroxysuccinimide Alanine Ester is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.