Description

Maleimide-NH-PEG4-CH2CH2COOH-PFP Ester is a heterobifunctional crosslinker featuring a maleimide group for thiol conjugation and a pentafluorophenyl (PFP) ester for efficient amine coupling. This compound is critical for creating stable, site-specific bioconjugates with high efficiency and minimal side-reactions. It is primarily utilized by researchers and manufacturers in the fields of biopharmaceutical development, antibody-drug conjugate (ADC) synthesis, and advanced diagnostic assay formulation.

Application

• Antibody-Drug Conjugate (ADC) Linker: Site-specific conjugation of cytotoxic payloads to monoclonal antibodies via cysteine residues.
• Protein and Peptide Labeling: Modification of thiol-containing biomolecules with fluorescent dyes, enzymes, or other probes via the amine-reactive PFP ester.
• Surface Functionalization: Immobilization of proteins or peptides onto amine-coated biosensors, chips, or nanoparticles.
• Polymer-Protein Conjugation: Synthesis of PEGylated proteins to enhance solubility, stability, and pharmacokinetic profiles.
• Diagnostic Reagent Development: Creation of stable immunoconjugates for ELISA, lateral flow assays, and other diagnostic platforms.
• Biomaterial Engineering: Crosslinking agent for creating hydrogels or functionalized matrices with controlled biological activity.

Basic Information

Item	Details
Product Name	Maleimide-NH-PEG4-CH2CH2COOH-PFP Ester
CAS No.	1347750-84-8
Molecular Formula	C27H32F5N3O10
Molecular Weight	653.56 g/mol
Synonyms	Maleimide-PEG4-PFP ester; PFP-PEG4-Maleimide; 4-((2,3,5,6-Tetrafluoro-4-(2,3,5,6-tetrafluorophenoxy)benzoyl)oxy)-2,5,8,11-tetraoxatridecan-13-oic acid 2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl ester; 2,3,5,6-Tetrafluoro-4-(2,3,5,6-tetrafluorophenoxy)benzoic acid PEG4 Maleimide ester; NHS-PEG4-Maleimide (PFP variant); Pentafluorophenyl 4-((17-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-3,6,9,12-tetraoxa-16-azaoctadecan-1-oyl)oxy)-2,3,5,6-tetrafluorobenzoate; Heterobifunctional PEG crosslinker, Maleimide and PFP ester terminated.
EINECS	Contact for details

Quality Control

Every batch of Maleimide-NH-PEG4-CH2CH2COOH-PFP Ester is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure product identity, purity, and performance for critical bioconjugation applications. A Certificate of Analysis (COA) detailing purity by HPLC, NMR confirmation, and other relevant specifications is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.