Description

Rac 4-Hydroxy-9-cis-Retinoic Acid-D3 is a deuterium-labeled isotopic analog of 9-cis-retinoic acid, specifically deuterated at three positions to enable precise quantitative tracking in pharmacokinetic and metabolic studies. This compound serves as a critical internal standard in LC-MS/MS bioanalysis for retinoid pathway research, offering enhanced mass resolution and reduced background interference compared to non-deuterated counterparts. It is essential for pharmaceutical R&D laboratories, contract research organizations (CROs), and academic institutions conducting mechanistic studies on nuclear receptor signaling, dermatological therapeutics, and developmental biology.

Application

• Internal standard for quantitative LC-MS/MS analysis of endogenous 9-cis-retinoic acid in plasma, tissue, and cell lysates
• Metabolic stability assessment in human and animal hepatocyte assays
• Pharmacokinetic (PK) and absorption, distribution, metabolism, and excretion (ADME) studies during retinoid drug development
• Calibration reference material for clinical biomarker validation in dermatology and oncology trials
• Tool compound for investigating RAR/RXR heterodimer activation kinetics in vitro
• Quality control standard for retinoid-based topical formulations requiring isotopic traceability
• Reference material for method validation per ICH M10 and FDA Bioanalytical Method Validation Guidance

Basic Information

Product Name	Rac 4-Hydroxy-9-cis-Retinoic Acid-D3
CAS No.	1346606-78-7
Molecular Formula	C20H25D3O2
Molecular Weight	305.43 g/mol
Synonyms	4-Hydroxy-9-cis-retinoic acid-d3; (all-E)-4-Hydroxy-9-cis-retinoic acid-d3; 4-Hydroxy-9-cis-retinoic acid-3,3,3-d3; 4-Hydroxy-9-cis-retinoic acid-2H3; Retinoic acid-d3 (9-cis, 4-hydroxy); 4-OH-9-cis-RA-d3; (±)-4-Hydroxy-9-cis-retinoic acid-d3; Rac-4-Hydroxy-9-cis-retinoic acid-3,3,3-d3
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for isotopic enrichment (>98% D-incorporation at designated positions), chemical purity (≥98.5% by HPLC-UV), identity (NMR and HRMS confirmation), and absence of critical impurities including residual solvents and heavy metals. Testing adheres to ICH Q5, Q7, and USP <1225> guidelines for reference standards used in regulated bioanalysis.

Storage

Preserve in a tightly closed container, protected from light. Store at −20 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Keep desiccated; avoid repeated freeze-thaw cycles. Handle under low-light conditions using amber vials and aluminum foil wrapping.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Retention time matches reference standard
Identification (HRMS)	m/z 306.2372 [M+H]+ (calcd for C20H26D3O2: 306.2371)
Assay (HPLC-UV)	≥98.5%
Isotopic Purity (D-incorporation)	≥98% at C-3, C-3′, C-3″ positions (by 2H-NMR)
Related Substances	≤0.5% total (each ≤0.1%)
Residual Solvents	Meets ICH Q3C limits
Water Content (KF)	≤0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.