Description

Spdp-Peg4-Nhs Ester is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a sulfhydryl-reactive pyridyldithiol group, connected by a flexible, hydrophilic tetraethylene glycol (PEG4) spacer. This molecular architecture is critical for creating stable, site-specific conjugates between proteins, peptides, and other biomolecules while maintaining solubility and reducing steric hindrance. It is an essential tool for researchers and developers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay manufacturing, where controlled linkage and payload delivery are paramount.

Application

• Antibody-Drug Conjugate (ADC) Linker: Site-specific conjugation of cytotoxic drugs to monoclonal antibodies via cysteine residues.
• Protein-Protein/Peptide Conjugation: Crosslinking amines and thiols on different biomolecules for creating bispecific constructs or fusion proteins.
• Surface Functionalization: Immobilization of proteins or peptides onto biosensor chips, nanoparticles, or chromatography resins via amine or thiol groups.
• Fluorescent/Enzyme Labeling: Attaching reporter molecules (e.g., fluorophores, enzymes) to antibodies or other proteins for diagnostic and imaging applications.
• PEGylation Reagent: Introducing a discrete, short PEG chain to biomolecules to improve solubility, stability, and pharmacokinetic properties.
• Biomaterial Modification: Functionalizing hydrogels or polymer scaffolds to create bioactive materials for tissue engineering and drug delivery.

Basic Information

Product Name	Spdp-Peg4-Nhs Ester
CAS No.	1334177-95-5
Molecular Formula	C27H39N3O11S2
Molecular Weight	645.74 g/mol
Synonyms	SPDP-PEG4-NHS ester; NHS-PEG4-SPDP; N-Succinimidyl 3-((2-(2-(2-(2-(3-(2-pyridyldithio)propanamido)ethoxy)ethoxy)ethoxy)ethyl)carbamoyl)propanoate; Succinimidyl-[(N-maleimidopropionamido)-tetraethyleneglycol] ester; PEG4-SPDP crosslinker; Heterobifunctional PEG crosslinker, NHS-PEG4-PDP; 3-(2-Pyridyldithio)-N-[2-[2-[2-[2-[[1-oxo-2,5-dihydro-1H-pyrrol-3-yl)oxy]carbonylamino]ethoxy]ethoxy]ethoxy]ethyl]propanamide
EINECS	Contact for details

Quality Control

Our Spdp-Peg4-Nhs Ester is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive bioconjugation applications. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm identity, purity, and functional integrity. Certificates of Analysis (COA) with detailed chromatographic data are provided, supporting development under GMP/GLP guidelines for preclinical and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or oily solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	Conjugation efficiency confirmed by model reaction

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.