Description

Biotin-Peg7-Azide is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation and click chemistry applications. This compound is critical for researchers and developers requiring precise, stable, and site-specific labeling of biomolecules, such as proteins and antibodies, with biotin tags. It is an essential tool for professionals in pharmaceutical R&D, proteomics, diagnostic assay development, and advanced materials science who need to create sophisticated detection and purification systems.

Application

• Antibody-Drug Conjugate (ADC) Development: For the site-specific conjugation of cytotoxic payloads to monoclonal antibodies via copper-free click chemistry.
• Diagnostic Assay Reagent: Used to biotinylate proteins, peptides, or oligonucleotides for use in ELISA, lateral flow assays, and other immunoassay platforms.
• Proteomics & Biomarker Discovery: Enables the selective labeling and subsequent pull-down of target proteins or protein complexes for mass spectrometry analysis.
• Cell Surface Engineering: Facilitates the modification of cell membranes with biotin groups for studying cell interactions, signaling, and for cell sorting applications.
• Nanoparticle Functionalization: A key linker for attaching targeting ligands (e.g., antibodies, peptides) and imaging agents to the surface of nanoparticles.
• Hydrogel and Biomaterial Synthesis: Serves as a crosslinker in the development of functionalized hydrogels for 3D cell culture and drug delivery systems.
• Affinity Chromatography Resin Production: Used to immobilize ligands onto solid supports for the purification of biomolecules.

Basic Information

Product Name	Biotin-Peg7-Azide
CAS No.	1334172-75-6
Molecular Formula	C33H60N8O11S
Molecular Weight	752.95 g/mol
Synonyms	Biotin-PEG7-N3; Biotin-PEG7-Azide; Biotinylated PEG Azide; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2-(2-(2-(2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethoxy)ethyl)pentanamide; Azido-PEG7-Biotin; N3-PEG7-Biotin; Biotin-azide PEG linker
EINECS	Contact for details

Quality Control

Our Biotin-Peg7-Azide is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm identity, purity, and structure. We provide a comprehensive Certificate of Analysis (COA) with each shipment, detailing all critical quality attributes. Our production adheres to cGMP guidelines where applicable, supporting its use in pre-clinical and diagnostic development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store under an inert atmosphere (e.g., argon or nitrogen) to maintain stability. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.