Description

3-(4-(n-Biotinoyl-6-Aminocaproyloxy)Phenyl)Propionic Acid, n-Hydroxysuccinimide Ester is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is critically important for its ability to introduce biotin tags onto target molecules via its amine-reactive NHS ester group, enabling subsequent capture or detection with streptavidin/avidin systems. It is an essential tool for researchers and manufacturers in biopharmaceutical development, diagnostic assay production, and proteomics research, where precise labeling and purification are paramount.

Application

• Biotinylation of Antibodies and Proteins: Primary application for creating biotin-labeled probes used in ELISA, Western blotting, and flow cytometry.
• Surface Functionalization for Biosensors: Immobilization of biomolecules on chips, beads, or plates for diagnostic and research platforms.
• Affinity Chromatography Ligand Synthesis: Preparation of biotinylated ligands for capture on streptavidin/avidin columns.
• Pull-Down Assays and Protein Interaction Studies: Tagging bait proteins to isolate interacting partners from complex biological mixtures.
• Diagnostic Kit Manufacturing: Key reagent in the production of lateral flow assays and other point-of-care diagnostic devices.
• Cell Surface Labeling: For tracking and isolating specific cell populations based on surface marker expression.
• Nucleic Acid Probe Labeling: Biotinylation of oligonucleotides for hybridization-based detection systems.

Basic Information

Product Name	3-(4-(n-Biotinoyl-6-Aminocaproyloxy)Phenyl)Propionic Acid, n-Hydroxysuccinimide Ester
CAS No.	1322623-44-8
Molecular Formula	C30H40N4O8S
Molecular Weight	616.73 g/mol
Synonyms	Biotin-XX, SSE; NHS-LC-LC-Biotin; Biotin-(AC5)2-Osu; EZ-Link NHS-LC-LC-Biotin; (+)-Biotinyl-6-aminocaproic acid N-hydroxysuccinimide ester; 6-((6-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoyl)oxy)-2,5-dioxopyrrolidin-1-yl 3-(4-hydroxyphenyl)propanoate; Succinimidyl 6-((6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoyl)oxy)-2,5-dioxopyrrolidin-1-yl 3-(4-hydroxyphenyl)propanoate
EINECS	Contact for details

Quality Control

This high-value biochemical reagent is manufactured under strict quality controls to ensure lot-to-lot consistency and optimal performance in sensitive applications. Each batch is characterized by HPLC, NMR, and mass spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment. Our quality system supports compliance with relevant standards for research and in-vitro diagnostic (IVD) manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and the active NHS ester group is susceptible to hydrolysis. For long-term stability, store desiccated. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 90.0% (on anhydrous basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.