Description

Brimonidine-D4 D-Tartrate CAS NO 1316758-27-6 is a high-purity, stable isotope-labeled analog of the α-2 adrenergic receptor agonist brimonidine, specifically deuterated at four positions. This compound is an essential internal standard for the precise and accurate quantification of brimonidine in complex biological matrices using advanced analytical techniques like LC-MS/MS. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical testing facilities engaged in pharmacokinetic studies, bioequivalence testing, and rigorous quality control of ophthalmic formulations.

Application

• Internal Standard for Bioanalysis: Serves as a critical deuterated internal standard in the quantitative LC-MS/MS analysis of brimonidine in plasma, serum, and other biological fluids.
• Pharmacokinetic & Metabolism Studies: Enables accurate tracking of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research.
• Bioequivalence Testing: Used to validate analytical methods for comparing the performance of generic and branded brimonidine formulations.
• Method Development & Validation: Essential for developing robust, sensitive, and specific analytical methods in compliance with FDA, EMA, and ICH guidelines.
• Quality Control & Impurity Profiling: Supports the identification and quantification of brimonidine and related substances in active pharmaceutical ingredients (APIs) and finished drug products.
• Stable Isotope Labeling Studies: Facilitates research into drug metabolism pathways and the differentiation of exogenous from endogenous compounds.

Basic Information

Product Name	Brimonidine-D4 D-Tartrate
CAS No.	1316758-27-6
Molecular Formula	C11H6D4BrN5 • C4H6O6
Molecular Weight	466.23 g/mol (approx.)
Synonyms	5-Bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-2,3,5,6-tetradeuteroquinoxalin-6-amine (2R,3R)-2,3-dihydroxybutanedioate; Brimonidine-d4 Tartrate; Brimonidine (D4) Tartrate Salt; Deuterated Brimonidine Tartrate; UK-14304-d4 Tartrate; Alphagan-d4 Internal Standard
EINECS	Contact for details

Quality Control

Our Brimonidine-D4 D-Tartrate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including NMR (¹H, ²H, 13C) for structural and isotopic confirmation, HPLC for chemical purity, and LC-MS to verify isotopic enrichment and absence of unlabeled compound. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes batch-specific data on purity, isotopic purity, and residual solvents, ensuring compliance with the stringent requirements of analytical and pharmaceutical research.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This material is hygroscopic (moisture-sensitive) and must be kept in a dry environment. For long-term storage, consider desiccant in the container. Keep the container tightly sealed when not in use to prevent absorption of moisture and degradation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Isotopic Purity (LC-MS)	≥ 98.0 atom % D
Chemical Purity	≥ 95.0% (by HPLC)
Residual Solvents (GC)	Complies with ICH Q3C guidelines
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.