Description

Mal-Amido-Peg5-C2-NHS Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring a maleimide group and an N-hydroxysuccinimide (NHS) ester, designed for precise bioconjugation. This compound is critical for creating stable, site-specific linkages between thiol-containing molecules (like cysteine residues in proteins or peptides) and primary amines, enabling the development of advanced biotherapeutics and diagnostics. It is an essential reagent for researchers and manufacturers in biopharmaceutical development, antibody-drug conjugate (ADC) production, and diagnostic assay kit formulation.

Application

• Antibody-Drug Conjugate (ADC) Linker: For the controlled attachment of cytotoxic payloads to monoclonal antibodies via thiol-maleimide chemistry.
• Protein/Peptide PEGylation: To conjugate PEG chains to therapeutic proteins or peptides, improving solubility, stability, and circulation half-life.
• Diagnostic Probe Conjugation: For labeling antibodies, proteins, or oligonucleotides with fluorophores, enzymes, or other detection tags.
• Surface Functionalization: To modify nanoparticles, biosensors, or microarray surfaces with bioactive molecules for targeted interactions.
• Hydrogel Formation: As a crosslinking agent in the synthesis of biomimetic hydrogels for tissue engineering and drug delivery systems.
• Biomolecule Immobilization: For the oriented and covalent immobilization of proteins or peptides onto solid supports for affinity chromatography or assay development.

Basic Information

Product Name	Mal-Amido-Peg5-C2-NHS Ester
CAS No.	1315355-92-0
Molecular Formula	C31H51N3O14
Molecular Weight	689.75 g/mol
Synonyms	Maleimide-PEG5-NHS Ester; NHS-PEG5-Maleimide; α-Maleimido-ω-NHS-PEG5; N-(2-{2-[2-(2-{3-[(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)methoxy]propanamido}ethoxy)ethoxy]ethoxy}ethoxy)ethyl)-2,5-dioxopyrrolidine-1-carboxamide; 1-[3-({2-[2-(2-{2-[2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethoxy]ethoxy}ethoxy)ethoxy]ethoxy}carbonyl)propyl]-1H-pyrrole-2,5-dione NHS ester; MAL-PEG5-NHS; Heterobifunctional PEG linker, Maleimide-PEG-NHS
EINECS	Contact for details

Quality Control

Our Mal-Amido-Peg5-C2-NHS Ester is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive bioconjugation workflows. Each lot undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and functional group assay. Certificates of Analysis (COA) with detailed specifications are provided to support your regulatory and R&D documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. Under these conditions, the product is stable for extended periods. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Assay (Maleimide)	≥ 90%
Functional Assay (NHS Ester)	≥ 90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.