Description

5-Methyl-4'-Thiocytidine is a chemically modified nucleoside analog where the oxygen atom at the 4' position of the ribose sugar is replaced by sulfur. This structural modification is of significant interest in medicinal chemistry and biochemistry research, particularly for its potential to alter the biological activity and metabolic stability of oligonucleotides. It serves as a critical building block for the synthesis of modified RNA and DNA strands. This compound is primarily utilized by researchers and developers in the fields of pharmaceutical development, antisense therapy, and molecular biology.

Application

• Antisense Oligonucleotide (ASO) Synthesis: A key monomer for creating therapeutic oligonucleotides with enhanced nuclease resistance and binding affinity.
• siRNA and miRNA Research: Used to incorporate modifications into small interfering RNA (siRNA) or microRNA (miRNA) to study gene silencing mechanisms and improve stability.
• Medicinal Chemistry & Drug Discovery: Serves as a precursor or intermediate in the development of novel nucleoside-based antiviral or anticancer agents.
• Biochemical Probe Development: Employed to create labeled or modified nucleotides for studying nucleic acid-protein interactions and enzyme mechanisms.
• Structural Biology Studies: Used to investigate the effect of sugar-phosphate backbone modifications on the structure and function of nucleic acids.

Basic Information

Product Name	5-Methyl-4'-Thiocytidine
CAS No.	1312295-87-6
Molecular Formula	C10H15N3O4S
Molecular Weight	273.31 g/mol
Synonyms	4'-Thio-5-methylcytidine; 5-Methylcytidine, 4'-thio-; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)-4-thiolan-2-yl)-5-methylpyrimidine-2,4-dione; 4'-Thio-5-methyl-C; 5-Methyl-4'-thio-C
EINECS	Contact for details

Quality Control

Our 5-Methyl-4'-Thiocytidine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification. We provide Certificates of Analysis (COA) with detailed specifications for every shipment, ensuring traceability and compliance with research-grade standards. Custom synthesis and purification to meet specific project requirements are available.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.