Description

Biotin-PEG4-Azide is a heterobifunctional crosslinker that combines the high-affinity biotin-streptavidin interaction with the bioorthogonal reactivity of an azide group via a tetraethylene glycol (PEG4) spacer. This compound is essential for site-specific bioconjugation, particularly in click chemistry applications, enabling the labeling, immobilization, and pull-down of biomolecules. It is a critical reagent for researchers and developers in biopharmaceutical R&D, proteomics, diagnostics, and materials science who require precise and stable conjugation strategies.

Application

• Bioconjugation and Probe Development: Site-specific labeling of proteins, antibodies, or oligonucleotides with biotin for detection or immobilization via copper-free click chemistry.
• Affinity Purification: Functionalization of solid supports (e.g., beads, resins) to create streptavidin-capture systems for isolating target molecules.
• Diagnostic Assays: Development of ELISA, lateral flow assays, or other biosensors by conjugating detection molecules.
• Cell Surface Engineering: Metabolic labeling and subsequent tagging of cell surface glycans or other biomolecules for imaging or sorting.
• ADC (Antibody-Drug Conjugate) Research: As a tool linker in the exploration of novel conjugation strategies for targeted drug delivery.
• Materials Science: Surface modification of nanomaterials (e.g., quantum dots, gold nanoparticles) to introduce biotin functionality for biomedical applications.
• Pull-Down Assays: Synthesis of activity-based probes for target identification and validation in chemical proteomics.

Basic Information

Product Name	Biotin-PEG4-Azide
CAS No.	1309649-57-7
Molecular Formula	C23H41N7O7S
Molecular Weight	559.68 g/mol
Synonyms	Biotin-PEG4-N3; Biotin-(PEG)4-azide; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethyl)pentanamide; Azido-PEG4-biotin; N-(Azido-PEG4)-biotin; Biotin tetraethylene glycol azide; Biotin-PEG4-N3
EINECS	Contact for details

Quality Control

Our Biotin-PEG4-Azide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure identity, potency, and consistency. Certificates of Analysis (COA) with detailed specifications are provided for every shipment, supporting your research, development, and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.