Description

trans-Sulfo-SMCC is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a sulfhydryl-reactive maleimide group, with a hydrophilic sulfonate group incorporated into the spacer arm. This compound is essential for creating stable, non-cleavable conjugates between proteins, peptides, or other biomolecules, particularly in aqueous environments where solubility is critical. It is a vital tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, diagnostic assay production, and biomaterials engineering.

Application

• Antibody-Drug Conjugate (ADC) Linker: Conjugating cytotoxic drugs to monoclonal antibodies via lysine residues on the antibody and thiol groups on the drug or linker-payload.
• Protein-Protein/Peptide Conjugation: Creating stable complexes for structural studies, immunoassays, or generating bispecific antibodies.
• Enzyme and Protein Immobilization: Covalently attaching biomolecules to solid supports (e.g., beads, plates, sensors) for diagnostics, affinity purification, or biosensor development.
• Fluorescent and Biotin Labeling: Site-specifically labeling proteins or antibodies with dyes, enzymes, or biotin for imaging (e.g., fluorescence microscopy) and detection (e.g., ELISA, flow cytometry).
• Polymer and Biomaterial Functionalization: Modifying hydrogels, nanoparticles, or other polymeric materials with bioactive ligands to enhance targeting and cellular interaction.
• Vaccine Development: Coupling antigens to carrier proteins to enhance immunogenicity.

Basic Information

Product Name	trans-Sulfo-SMCC
CAS No.	1286837-77-1
Molecular Formula	C16H17N2NaO10S2
Molecular Weight	500.43 g/mol
Synonyms	4-((4-(((2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)oxy)carbonyl)cyclohexyl)methoxy)-4-oxobutane-1-sulfonic Acid Sodium Salt; Sodium 4-((4-((2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)oxy)carbonyl)cyclohexyl)methoxy)-4-oxobutane-1-sulfonate; Sulfo-SMCC (trans isomer); Sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC); SMCC Sulfonate Sodium Salt; NHS-PEG4-Maleimide (sulfo, trans-cyclohexyl); Heterobifunctional Crosslinker, Sulfo-SMCC.
EINECS	Contact for details

Quality Control

Our trans-Sulfo-SMCC is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent performance, and compliance with relevant standards for research and bioconjugation applications. A comprehensive Certificate of Analysis (COA) detailing purity (by HPLC), identity (by NMR/MS), and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water, DMSO
Functional Activity	Conforms (by conjugation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.