Description

n-Acetyl Tizanidine-D4 is a deuterated internal standard of the acetylated metabolite of Tizanidine, a centrally acting muscle relaxant. This high-purity, isotopically labeled compound is essential for ensuring the accuracy and reliability of quantitative bioanalytical methods, particularly Liquid Chromatography-Mass Spectrometry (LC-MS). It is primarily used by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical testing facilities for pharmacokinetic studies, drug metabolism research, and therapeutic drug monitoring.

Application

• Quantitative Bioanalysis: Serves as a critical internal standard for the precise LC-MS/MS quantification of Tizanidine and its metabolites in biological matrices (plasma, serum, urine).
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Enables accurate measurement of drug concentration-time profiles to determine absorption, distribution, metabolism, and excretion.
• Drug Metabolism and Disposition (DMPK): Used in metabolite profiling and identification studies to understand the biotransformation pathways of Tizanidine.
• Clinical Research & Therapeutic Drug Monitoring (TDM): Supports the development and validation of robust assays for monitoring patient drug levels to optimize dosing and efficacy.
• Reference Standard for Impurity Testing: Can be utilized as a reference material in the analysis of related substances and degradation products in pharmaceutical formulations.

Basic Information

Product Name	n-Acetyl Tizanidine-D4
CAS No.	1276602-33-5
Molecular Formula	C₁₀H₄D₄ClN₅OS
Molecular Weight	277.75 g/mol
Synonyms	5-Chloro-N-(4,5-dihydro-1H-imidazol-2-yl)-2,1,3-benzothiadiazol-4-amine-d4 Acetamide; Tizanidine N-Acetyl Metabolite-d4; Tizanidine Acetamide-d4; 2-Acetamido-4-(2-amino-4,5-dihydro-1H-imidazol-4-yl)-5-chloro-2,1,3-benzothiadiazole-d4; Deuterated n-Acetyl Tizanidine; Tizanidine-d4 Acetamide; Internal Standard for Tizanidine; [²H₄]-n-Acetyl Tizanidine
EINECS	Contact for details

Quality Control

Every batch of n-Acetyl Tizanidine-D4 is manufactured and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, supported by comprehensive analytical data including NMR, HPLC, and MS. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity (by HPLC), chemical purity, isotopic enrichment (D%), and other critical parameters to meet the stringent requirements of analytical and regulatory standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and must be kept under inert atmosphere or desiccated conditions after opening to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Chemical Purity	≥ 95.0%
Isotopic Purity (D%)	≥ 98.0 atom % D
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.