Description

4-Hydroxy Midazolam-D5 Methanoate is a deuterated internal standard of a key midazolam metabolite, essential for precise quantitative analysis in complex biological matrices. Its primary value lies in providing unmatched accuracy and reliability for pharmacokinetic and forensic toxicology studies, where consistent results are critical. This high-purity reference material is indispensable for pharmaceutical R&D laboratories, clinical research organizations, and forensic science institutions conducting advanced LC-MS/MS analysis.

Application

• Internal Standard for LC-MS/MS: Primary application as a stable, isotopically-labeled internal standard for the accurate quantification of 1'-hydroxymidazolam and related metabolites in plasma, serum, and urine samples.
• Pharmacokinetic & Bioequivalence Studies: Enables precise measurement of metabolite concentrations over time to determine drug metabolism rates, half-life, and bioequivalence of midazolam formulations.
• Forensic Toxicology & DUID Analysis: Critical for reliable and court-defensible analysis in Driving Under the Influence of Drugs (DUID) cases and post-mortem toxicology to determine midazolam exposure.
• Drug Metabolism & Disposition Research: Used in in vitro and in vivo studies to elucidate the metabolic pathways and enzymatic activity related to midazolam.
• Clinical Trial Support: Supports analytical method development and validation for clinical trials involving midazolam as a sedative or anesthetic agent.
• Method Development & Validation: Serves as a critical tool for developing, optimizing, and validating robust, sensitive, and specific analytical methods in regulated bioanalytical laboratories.

Basic Information

Product Name	4-Hydroxy Midazolam-D5 Methanoate
CAS No.	1276584-44-1
Molecular Formula	C19H12D5ClFN3O3
Molecular Weight	390.83 g/mol (approximate)
Synonyms	8-Chloro-6-(2-fluorophenyl)-1-methyl-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylic acid, 4-hydroxy-, methyl ester-d5; 1'-Hydroxymidazolam-d5 Methyl Ester; 1'-OH-Midazolam-d5 Methyl Ester; Methyl 1'-hydroxymidazolam-d5 carboxylate; Deuterated 1'-Hydroxymidazolam Methyl Ester; Midazolam Metabolite D5 Internal Standard; α-Hydroxymidazolam-d5 Methyl Ester
EINECS	Contact for details

Quality Control

Every batch of 4-Hydroxy Midazolam-D5 Methanoate is manufactured under strict quality systems and undergoes comprehensive analytical testing to ensure identity, purity, and isotopic enrichment meet the exacting standards required for analytical reference materials. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes results from NMR, HPLC, and MS analysis. Our quality commitment supports compliance with GMP/GLP principles for use in regulated bioanalytical laboratories.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. Store in a cool, dry, and well-ventilated area.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Deuterium Enrichment)	≥ 98.0 atom % D
Chemical Purity	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.