Description

n-Acetyl-D3-L-Leucine-D10-n-Methyl-D3-Amide is a high-purity, multi-deuterated isotopologue of the acetylated leucine derivative, specifically designed for advanced research and analytical applications. This compound is critical for use as an internal standard in mass spectrometry, enabling precise quantification and metabolic pathway tracing in complex biological matrices. It is primarily utilized by pharmaceutical researchers, contract research organizations (CROs), and analytical laboratories engaged in drug metabolism and pharmacokinetics (DMPK) studies, proteomics, and stable isotope labeling.

Application

• Internal Standard for LC-MS/MS: Essential for accurate quantitative bioanalysis in drug development and clinical research.
• Metabolic Pathway Tracing: Used in stable isotope labeling studies to investigate amino acid metabolism and protein turnover.
• Proteomics Research: Serves as a labeled standard for mass spectrometry-based protein and peptide quantification.
• Pharmaceutical Reference Standard: Employed for method development, validation, and quality control in analytical laboratories.
• Biochemical Research: A tool for studying enzyme kinetics and mechanisms involving leucine derivatives.
• Nutritional Science Studies: Applied in research on protein metabolism and amino acid utilization.

Basic Information

Product Name	n-Acetyl-D3-L-Leucine-D10-n-Methyl-D3-Amide
CAS No.	1276197-56-8
Molecular Formula	C9D16H3N2O2
Molecular Weight	~207.3 g/mol (deuterated form)
Synonyms	N-Acetyl-D3-L-leucine-D10-N-methyl-D3-amide; Acetyl-D3-leucine-D10-methyl-D3-amide; Deuterated N-Acetyl-L-leucine N-methylamide; [D16]-N-Acetyl-N-methyl-L-leucinamide; L-Leucine, N-acetyl-N-methyl-, D16 isotopologue; Stable Isotope Labeled Leucine Derivative; MS Internal Standard 1276197-56-8
EINECS	Contact for details

Quality Control

Our n-Acetyl-D3-L-Leucine-D10-n-Methyl-D3-Amide is manufactured under strict quality systems to ensure the highest isotopic and chemical purity required for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (D, atom %), chemical purity, and identity confirmation. We adhere to relevant industry guidelines for reference standard materials to ensure reliable and reproducible performance in your assays.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This material is hygroscopic (moisture-sensitive); desiccate upon opening and keep under an inert atmosphere for long-term storage to maintain stability and isotopic integrity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (D, atom %)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.