Description

2,5-Dioxopyrrolidin-1-Yl 1-(3-(2,5-Dioxo-2,5-Dihydro-1H-Pyrrol-1-Yl)Phenyl)-1-Oxo-5,8,11,14-Tetraoxa-2-Azaheptadecan-17-Oate is a specialized heterobifunctional crosslinking reagent featuring both an NHS ester and a maleimide group, connected by a hydrophilic polyethylene glycol (PEG) spacer. This molecular architecture is designed for the site-specific conjugation of biomolecules, enabling the creation of stable amide bonds with primary amines and thioether bonds with free thiols. It is an essential tool for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and advanced diagnostics, where precise and stable linker chemistry is paramount for product efficacy and consistency.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key intermediate for constructing ADCs, connecting cytotoxic payloads to monoclonal antibodies via stable linkages.
• Protein and Peptide Labeling: For site-specific attachment of fluorophores, biotin, or other probes to proteins for imaging, detection, and assay development.
• Surface Functionalization: Used to modify nanoparticles, biosensors, or chromatography matrices with bioactive ligands for targeted binding.
• Polymer-Protein Conjugation: Enables the PEGylation of therapeutic proteins to enhance solubility, stability, and circulation half-life.
• Diagnostic Reagent Synthesis: Manufacture of enzyme conjugates and other critical components for immunoassay kits (ELISA, lateral flow).
• Biomaterial Engineering: Crosslinking agent for creating hydrogels or functional biomaterials with tailored biological activity.
• Small Molecule-Protein Conjugation: Linking haptens to carrier proteins for antibody production.

Basic Information

Product Name	2,5-Dioxopyrrolidin-1-Yl 1-(3-(2,5-Dioxo-2,5-Dihydro-1H-Pyrrol-1-Yl)Phenyl)-1-Oxo-5,8,11,14-Tetraoxa-2-Azaheptadecan-17-Oate
CAS No.	1263044-88-7
Molecular Formula	C28H35N3O12
Molecular Weight	605.60 g/mol
Synonyms	NHS-PEG4-Maleimide; Maleimide-PEG4-NHS ester; SM(PEG)4; 1-(3-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)phenyl)-1-oxo-5,8,11,14-tetraoxa-2-azaheptadecan-17-oic acid 2,5-dioxopyrrolidin-1-yl ester; N-Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate-PEG4; Heterobifunctional PEG crosslinker, NHS-PEG4-MAL; PEG-based crosslinking reagent
EINECS	Contact for details

Quality Control

Every batch of our 2,5-Dioxopyrrolidin-1-Yl 1-(3-(2,5-Dioxo-2,5-Dihydro-1H-Pyrrol-1-Yl)Phenyl)-1-Oxo-5,8,11,14-Tetraoxa-2-Azaheptadecan-17-Oate is manufactured under strict quality management systems. We provide comprehensive analytical data to ensure high purity and batch-to-batch consistency, critical for sensitive bioconjugation applications. Certificates of Analysis (COA) detailing purity (HPLC), identity (NMR, MS), and functional group integrity are available upon request. Our production adheres to cGMP guidelines where applicable, supporting its use in pharmaceutical research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis. For long-term storage, keep under an inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.