Description

Biotin-Peg4-Alkyne CAS NO 1262681-31-1 is a high-purity, heterobifunctional crosslinking reagent that combines the strong binding affinity of biotin with a polyethylene glycol (PEG) spacer and a terminal alkyne group for bioorthogonal click chemistry. This compound is essential for researchers and manufacturers requiring precise, stable, and site-specific conjugation of biomolecules, such as proteins, antibodies, and nucleic acids, to various surfaces or detection systems. It is a critical tool for professionals in bioconjugation, proteomics, drug discovery, and diagnostic assay development, enabling advanced research and the creation of sensitive detection platforms.

Application

• Bioconjugation and Probe Development: Site-specific labeling of proteins, antibodies, and peptides for use in pull-down assays, western blotting, and ELISA.
• Proteomics and Interactome Analysis: Immobilization of bait proteins onto streptavidin-coated surfaces for the study of protein-protein interactions.
• Diagnostic Assay Fabrication: Conjugation of detection molecules in lateral flow assays, biosensors, and other immunoassay formats.
• Drug Discovery & ADC Development: Facilitating the synthesis and screening of biotinylated small molecules or antibody-drug conjugates (ADCs) for target identification and validation.
• Cell Surface Engineering: Labeling of cell surface glycans or other biomolecules via click chemistry for imaging and tracking studies.
• Material Science & Nanotechnology: Functionalization of nanoparticles, quantum dots, or other nanomaterials with biological recognition elements.
• Affinity Chromatography: Preparation of biotinylated ligands for immobilization on streptavidin/avidin resins.
• DNA/RNA Labeling: Incorporation into oligonucleotides for the detection and purification of nucleic acid complexes.

Basic Information

Product Name	Biotin-Peg4-Alkyne
CAS No.	1262681-31-1
Molecular Formula	C29H49N5O8S
Molecular Weight	627.79 g/mol
Synonyms	Biotin-PEG4-Alkyne; Biotin-PEG4-Propargyl; 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2-(2-(2-(2-(prop-2-yn-1-yloxy)ethoxy)ethoxy)ethoxy)ethyl)pentanamide; Biotin-PEG4-Pentyne; Biotin-Polyethylene Glycol-Alkyne; Alkyne-PEG4-Biotin; Biotinylated PEG Alkyne; Biotin-PEG4-C≡CH
EINECS	Contact for details

Quality Control

Our Biotin-Peg4-Alkyne is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure identity, purity, and consistency. Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We support compliance with relevant research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.