Description

Midazolam-[13C6] is a stable isotope-labeled analog of the pharmaceutical compound Midazolam, where six carbon atoms are replaced with the Carbon-13 isotope. This product is essential as an internal standard in quantitative bioanalytical methods, ensuring the highest accuracy and reliability in mass spectrometry-based analyses. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and forensic toxicology labs for applications in drug metabolism, pharmacokinetic studies, and clinical research.

Application

• Quantitative Bioanalysis: Serves as a critical internal standard for the precise quantification of Midazolam and its metabolites in biological matrices (e.g., plasma, serum, urine) using LC-MS/MS and GC-MS.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in absorption, distribution, metabolism, and excretion (ADME) studies to track drug pathways with high specificity.
• Clinical Research & Therapeutic Drug Monitoring (TDM): Enables accurate measurement of drug concentrations in patient samples for dose optimization and safety studies.
• Forensic and Clinical Toxicology: Facilitates the definitive identification and quantification of Midazolam in complex forensic casework and overdose scenarios.
• Pharmaceutical Impurity Profiling: Can be utilized as a reference standard in the development and validation of methods for impurity identification.
• Isotope Dilution Mass Spectrometry (IDMS): Acts as the ideal spike-in standard for this gold-standard quantitative technique, correcting for analyte loss and instrument variability.

Basic Information

Product Name	Midazolam-[13C6]
CAS No.	1261396-95-5
Molecular Formula	C1713C6H13ClFN3
Molecular Weight	331.76 (for 13C6 labeled form)
Synonyms	8-Chloro-6-(2-fluorophenyl)-1-methyl-4H-13C6-imidazo[1,5-a][1,4]benzodiazepine; 13C6-Midazolam; Midazolam (phenyl-13C6); Midazolam-13C6; Stable Isotope-Labeled Midazolam; Midazolam Internal Standard; Dormicum-13C6; Versed-13C6
EINECS	Contact for details

Quality Control

Our Midazolam-[13C6] is manufactured under strict quality controls to ensure exceptional isotopic purity and chemical integrity, suitable for the most demanding analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically ≥99 atom % 13C), chemical purity (by HPLC), and confirmatory structural data. We adhere to relevant industry standards for reference materials, and custom synthesis to meet specific purity or concentration requirements is available.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label or COA. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (Mass Spectrometry)	Conforms
Purity (HPLC)	≥98.0%
Isotopic Enrichment	≥99 atom % 13C
Chemical Purity	≥95%
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.