Description

[13C6]-Hydrochlorothiazide is a stable isotope-labeled analog of the widely prescribed diuretic and antihypertensive agent, hydrochlorothiazide. This high-purity internal standard is critical for ensuring the accuracy and reliability of quantitative mass spectrometry analyses in pharmaceutical research and development. It is an essential tool for scientists and quality control professionals in pharmaceutical, contract research, and clinical diagnostic laboratories who require definitive methods for drug metabolism, pharmacokinetic studies, and bioanalytical method validation.

Application

• Internal Standard for LC-MS/MS: Primary application as a quantitative internal standard in liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the precise measurement of hydrochlorothiazide in biological matrices (plasma, serum, urine).
• Drug Metabolism & Pharmacokinetics (DMPK): Used in studies to track the absorption, distribution, metabolism, and excretion (ADME) of hydrochlorothiazide, enabling accurate metabolite profiling.
• Bioanalytical Method Development & Validation: Critical for developing and validating robust, sensitive, and specific analytical methods in compliance with FDA, EMA, and ICH guidelines.
• Clinical Research & Therapeutic Drug Monitoring (TDM): Supports clinical trials and TDM programs by providing accurate quantification of hydrochlorothiazide levels in patient samples.
• Pharmaceutical Quality Control (QC): Employed in impurity profiling and assay development to ensure the quality and consistency of active pharmaceutical ingredients (APIs) and finished drug products.
• Forensic & Anti-Doping Analysis: Used in specialized testing laboratories for the definitive identification and quantification of hydrochlorothiazide.

Basic Information

Product Name	[13C6]-Hydrochlorothiazide
CAS No.	1261396-79-5
Molecular Formula	C713C6H8ClN3O4S2
Molecular Weight	303.75 (for 13C6 labeled form)
Synonyms	Hydrochlorothiazide-13C6; 6-Chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide-13C6; HCTZ-13C6; Isotopically Labeled Hydrochlorothiazide; Stable Isotope-Labeled Hydrochlorothiazide; 13C6-HCTZ; 3,4-Dihydro-6-chloro-7-sulfamoyl-2H-1,2,4-benzothiadiazine 1,1-dioxide-13C6
EINECS	Contact for details

Quality Control

Our [13C6]-Hydrochlorothiazide is manufactured under strict quality systems to ensure the highest isotopic and chemical purity required for sensitive analytical applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically ≥99 atom % 13C), chemical purity (by HPLC), and confirming identity via spectroscopic methods. Our quality commitment supports compliance with the rigorous standards of GLP and GMP environments.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This compound is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which could affect stability and analytical performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment	≥99 atom % 13C
Chemical Purity	≥95% (by NMR)
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.