Description

N,o-Didesmethyl Tramadol-D3 Hydrochloride is a high-purity, deuterium-labeled internal standard critical for accurate quantitative analysis in pharmaceutical research and forensic toxicology. This compound is essential for ensuring the precision and reliability of mass spectrometry-based assays, particularly in method development and validation. It is primarily utilized by analytical laboratories, contract research organizations (CROs), and pharmaceutical companies engaged in pharmacokinetic studies, drug metabolism research, and compliance testing.

Application

• Internal Standard for LC-MS/MS Analysis: Used as a stable isotope-labeled reference compound for the precise quantification of tramadol and its metabolites in biological matrices.
• Pharmaceutical R&D: Supports method development and validation in drug metabolism and pharmacokinetics (DMPK) studies.
• Forensic and Clinical Toxicology: Enables accurate screening and confirmation of tramadol abuse or overdose in urine, blood, and plasma samples.
• Bioanalytical Method Validation: Critical for meeting regulatory requirements (e.g., FDA, EMA) for assay specificity, accuracy, and precision.
• Reference Material Manufacturing: Serves as a certified component in the production of quality control and proficiency testing materials.

Basic Information

Product Name	N,o-Didesmethyl Tramadol-D3 Hydrochloride
CAS No.	1261393-89-8
Molecular Formula	C15H20D3NO2•HCl
Molecular Weight	289.83 g/mol (Hydrochloride salt)
Synonyms	O-Desmethyl Tramadol-D3; N,O-Didesmethyl Tramadol-D3 HCl; Tramadol Metabolite M5-D3 Hydrochloride; 1-(3-Methoxy-4-[(2-[(methyl-d3)amino]methyl]cyclohexyl)phenol Hydrochloride; (+/-)-N,O-Didesmethyltramadol-d3 hydrochloride; Deuterated Tramadol Metabolite Standard; Tramadol-d3 Internal Standard; ISTD for Tramadol Assay
EINECS	Contact for details

Quality Control

Every batch of N,o-Didesmethyl Tramadol-D3 Hydrochloride is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure the highest standards of isotopic purity, chemical purity, and stability. Our quality assurance protocol includes comprehensive characterization by NMR, HPLC, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity (typically ≥95% by HPLC), isotopic enrichment (≥98 atom % D), and other critical specifications to support your regulatory submissions.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content (KF)	≤ 5.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.