Description

(+)-o-Desmethyl Tramadol-D6 is a deuterium-labeled internal standard of the primary active metabolite of tramadol. This compound is essential for ensuring the accuracy and reliability of quantitative analytical methods, particularly in regulated environments. It is primarily used by pharmaceutical R&D laboratories, forensic toxicology units, and contract research organizations (CROs) for precise pharmacokinetic studies, drug metabolism research, and bioanalytical method validation.

Application

• Bioanalytical Method Development and Validation: Serves as a critical internal standard in LC-MS/MS and GC-MS assays for quantifying tramadol and its metabolites in biological matrices like plasma, serum, and urine.
• Pharmacokinetic and Metabolism Studies: Enables accurate tracking of tramadol's metabolic pathway (O-demethylation) in preclinical and clinical research, providing essential data on drug absorption, distribution, metabolism, and excretion (ADME).
• Forensic and Clinical Toxicology: Used as a reference standard in confirmatory testing for tramadol use, overdose cases, and compliance monitoring, ensuring legally defensible results.
• Pharmaceutical Quality Control: Supports impurity profiling and stability testing of tramadol-based drug products by providing a reference for the O-desmethyl tramadol metabolite.
• Doping Control in Sports: Employed in anti-doping laboratories to accurately detect and quantify the presence of tramadol, which is prohibited in certain sports.

Basic Information

Product Name	(+)-o-Desmethyl Tramadol-D6
CAS No.	1261393-87-6
Molecular Formula	C15H19D6NO2
Molecular Weight	269.42 g/mol
Synonyms	(+)-O-Desmethyltramadol-D6; (+)-O-Demethyltramadol-D6; M1 Metabolite of Tramadol-D6; (1R,2R)-2-[(Dimethylamino)methyl]-1-(3-methoxyphenyl)cyclohexanol-D6; O-Desmethyl Tramadol-D6 (labeled); Tramadol Metabolite M1-D6; [2-[(Dimethylamino)methyl]-1-(3-methoxyphenyl)cyclohexanol-D6] (1R,2R)-form
EINECS	Contact for details

Quality Control

Every batch of (+)-o-Desmethyl Tramadol-D6 is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. We guarantee high chemical and isotopic purity verified by advanced techniques including NMR (¹H, ¹³C, ²H), High-Resolution Mass Spectrometry (HRMS), and HPLC. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, isotopic enrichment, and confirming the absence of unlabeled compound. Our standards are suitable for use in GLP/GMP-compliant laboratories.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This compound is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (²H NMR/MS)	≥ 98.0 atom % D
Chemical Purity	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.