Description

18-Hydroxycorticosterone-[D4] is a high-purity, deuterium-labeled internal standard critical for advanced analytical quantification. This stable isotope-labeled compound is essential for ensuring accuracy and precision in mass spectrometry-based assays by correcting for analyte loss during sample preparation and instrument variability. It is primarily required by pharmaceutical R&D laboratories, clinical research organizations, and contract testing facilities engaged in steroid hormone analysis, metabolomics studies, and the development of bioanalytical methods.

Application

• Internal Standard for LC-MS/MS: Used as a quantitative reference in liquid chromatography-tandem mass spectrometry for the precise measurement of endogenous 18-hydroxycorticosterone and related corticosteroids in biological matrices.
• Biomedical Research: Facilitates studies on adrenal steroidogenesis, hypertension research related to mineralocorticoid pathways, and investigations into disorders of the renin-angiotensin-aldosterone system.
• Clinical Diagnostics Development: Enables the development and validation of high-sensitivity clinical assays for hormone level monitoring in patient samples.
• Metabolomics and Biomarker Discovery: Serves as a stable isotope tracer in metabolic flux analysis and the identification of steroid-based biomarkers.
• Pharmaceutical Quality Control: Applied in the analysis of drug substances and products where corticosteroid impurities or related substances must be monitored.
• Forensic Toxicology: Supports confirmatory testing for endogenous steroid profiling in anti-doping and forensic investigations.

Basic Information

Product Name	18-Hydroxycorticosterone-[D4]
CAS No.	1257742-38-3
Molecular Formula	C21H26D4O5
Molecular Weight	368.50 g/mol (approximate)
Synonyms	18-Hydroxycorticosterone-d4; 11β,18,21-Trihydroxypregn-4-ene-3,20-dione-18,21,21,21-d4; 18-OH-Corticosterone-d4; 18-Hydroxy-B-d4; Deuterated 18-Hydroxycorticosterone; 18-Hydroxycorticosterone (tetradeuterated); 18-OHB-d4; 18-Hydroxycorticosterone Internal Standard
EINECS	Contact for details

Quality Control

Every batch of 18-Hydroxycorticosterone-[D4] is manufactured and analyzed under strict quality systems to ensure the highest isotopic and chemical purity required for sensitive analytical applications. Our products undergo rigorous quality testing, including NMR and mass spectrometry for isotopic enrichment verification and HPLC for chemical purity assessment. A comprehensive Certificate of Analysis (COA) detailing batch-specific results for purity, isotopic purity, and identity is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC-MS)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Isotopic Purity (by MS)	≥ 98.0 atom % D
Chemical Purity	≥ 95.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.