Description

Dbco-Peg4-Biotin is a heterobifunctional crosslinker that combines a dibenzocyclooctyne (DBCO) group for strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry with a biotin moiety for high-affinity streptavidin/avidin binding, connected by a tetraethylene glycol (PEG4) spacer. This molecular architecture is critical for creating stable, site-specific bioconjugates without the need for cytotoxic copper catalysts. It is an essential reagent for researchers and developers in bioconjugation, targeted drug delivery, diagnostic assay development, and advanced materials science.

Application

• Antibody-Drug Conjugate (ADC) Development: For the site-specific, copper-free conjugation of cytotoxic payloads to monoclonal antibodies via the DBCO handle, with subsequent purification or detection enabled by the biotin tag.
• Diagnostic Assay & Biosensor Fabrication: Used to functionalize surfaces (e.g., chips, beads, electrodes) with biomolecules for ELISA, lateral flow assays, or SPR-based detection systems.
• Cell Surface Labeling & Imaging: Enables the tagging of azide-modified glycans or other biomolecules on live cells for fluorescence microscopy or flow cytometry analysis via biotin-streptavidin amplification.
• Protein & Peptide Modification: Facilitates the biotinylation of azide-functionalized proteins or peptides for pull-down assays, affinity purification, or Western blot detection.
• Polymer & Nanomaterial Functionalization: Serves as a key building block for creating biofunctionalized hydrogels, nanoparticles, or dendrimers for targeted therapeutic and diagnostic applications.
• Chemical Biology & Proteomics: A vital tool for activity-based protein profiling (ABPP) and the study of protein-protein interactions through click chemistry-based labeling strategies.

Basic Information

Item	Detail
Product Name	Dbco-Peg4-Biotin
CAS No.	1255942-07-4
Molecular Formula	C44H60N4O9S
Molecular Weight	821.04 g/mol
Synonyms	DBCO-PEG4-Biotin; DBCO-PEG4-Biotin; Dibenzocyclooctyne-PEG4-Biotin; (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-{2-[2-{2-[2-(5-{2-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamido}ethoxy)ethoxy]ethoxy}ethoxy]ethyl}carbamate; DBCO-SS-PEG4-Biotin; DBCO-PEG4-Biotin Conjugate; Azadibenzocyclooctyne-PEG4-Biotin
EINECS	Contact for details

Quality Control

Our Dbco-Peg4-Biotin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with molecular structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.