Description

Biotin-PEG3-NHS Ester is a heterobifunctional crosslinker and bioconjugation reagent featuring a biotin moiety for affinity capture and a reactive NHS ester for stable amide bond formation with primary amines. This compound is essential for creating biotinylated probes, antibodies, and other biomolecules with a hydrophilic polyethylene glycol (PEG) spacer that reduces steric hindrance and improves solubility. It is a critical tool for researchers and manufacturers in pharmaceutical development, diagnostic assay production, and proteomics research, enabling precise labeling and purification workflows.

Application

• Labeling of antibodies, proteins, and peptides for use in ELISA, Western blotting, and flow cytometry.
• Surface functionalization of biosensors, microarrays, and nanoparticles for diagnostic applications.
• Preparation of biotinylated affinity probes for pull-down assays and mass spectrometry-based proteomics.
• Conjugation to oligonucleotides for nucleic acid detection and hybridization studies.
• Development of targeted drug delivery systems by linking therapeutic agents to targeting ligands.
• Immobilization of biomolecules on streptavidin/avidin-coated plates, beads, or chips.

Basic Information

Product Name	Biotin-PEG3-NHS Ester
CAS No.	1253286-56-4
Molecular Formula	C24H39N5O8S
Molecular Weight	557.66 g/mol
Synonyms	Biotin-PEG3-NHS ester; Biotinamido-PEG3-NHS ester; Biotin-(PEG)3-NHS ester; Biotin-PEG3-N-hydroxysuccinimide ester; Biotin-PEG3-OSu; Biotin-PEG3-SE; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethoxy]propanoate; EZ-Link Biotin-PEG3-NHS Ester
EINECS	Contact for details

Quality Control

Our Biotin-PEG3-NHS Ester is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance. We adhere to ISO 9001 quality management standards, and our processes are designed to meet the stringent requirements of GMP environments for critical bioconjugation workflows.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis of the active NHS ester group.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.