Description

Chlorhexidine-D8 Dihydrochloride is a deuterated analog of the widely used antiseptic agent chlorhexidine dihydrochloride, where eight hydrogen atoms are replaced with deuterium. This isotopic labeling makes it an essential internal standard for the precise quantification of chlorhexidine in complex biological matrices using advanced analytical techniques like LC-MS/MS. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical testing facilities engaged in method development, pharmacokinetic studies, and regulatory compliance testing for antiseptic formulations. The compound is supplied with guaranteed high chemical and isotopic purity to ensure reliable and reproducible analytical results.

Application

• Internal Standard for Bioanalysis: Serves as a critical internal standard in the LC-MS/MS quantification of chlorhexidine in plasma, serum, urine, and tissue homogenates for pharmacokinetic and toxicokinetic studies.
• Pharmaceutical R&D: Used in the development and validation of analytical methods for new antiseptic formulations, topical creams, oral rinses, and medical device coatings containing chlorhexidine.
• Doping Control & Forensic Analysis: Employed in specialized testing laboratories for the accurate detection and confirmation of chlorhexidine, which may be regulated in certain sports or forensic contexts.
• Metabolite Identification Studies: Aids in distinguishing drug-derived metabolites from endogenous compounds in mass spectrometry-based metabolism and degradation pathway studies.
• Quality Control in Manufacturing: Used as a reference standard in the in-process and final product quality control of chlorhexidine-based pharmaceutical products to ensure batch-to-batch consistency.
• Environmental and Residue Testing: Applicable in methods developed to trace chlorhexidine residues in environmental samples or on medical surfaces.

Basic Information

Product Name	Chlorhexidine-D8 Dihydrochloride
CAS No.	1246816-96-5
Molecular Formula	C22H22D8Cl2N10·2HCl
Molecular Weight	578.41 g/mol (for unlabeled form; exact mass varies with isotopic enrichment)
Synonyms	1,1'-Hexamethylenebis[5-(p-chlorophenyl)biguanide]-D8 Dihydrochloride; Chlorhexidine-d8 Dihydrochloride; Deuterated Chlorhexidine Dihydrochloride; N,N''''-Bis(4-chlorophenyl)-3,12-diimino-2,4,11,13-tetraazatetradecanediimidamide-D8 Dihydrochloride; 1,6-Bis(N5-p-chlorophenyl-N1-biguanidino)hexane-D8 Dihydrochloride; Chlorhexidine (D8) Dihydrochloride; Biguanide, 1,1'-hexamethylenebis[5-(4-chlorophenyl)-, D8, Dihydrochloride
EINECS	Contact for details

Quality Control

Every batch of our Chlorhexidine-D8 Dihydrochloride is manufactured and analyzed under strict quality management systems. We guarantee high chemical purity and specified isotopic enrichment (typically ≥98 atom % D) to meet the exacting requirements of analytical reference standards. Comprehensive testing includes identification (NMR, IR), assay (HPLC), determination of isotopic purity (MS), and control of related substances and residual solvents. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 98 atom % D
Chemical Purity (by NMR)	≥ 95%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.