Description

6α-Hydroxy-5α-Cholestane-D7 is a high-purity, deuterium-labeled sterol derivative, specifically designed for use as an internal standard in advanced analytical chemistry. This compound is critical for ensuring the accuracy and reliability of quantitative analyses in complex biological matrices, where precise measurement is paramount. It is primarily utilized by research institutions, pharmaceutical developers, and contract research organizations (CROs) engaged in lipidomics, metabolic studies, and drug development.

Application

• Internal Standard for Mass Spectrometry (MS): Essential for the precise quantification of cholesterol and its metabolites in LC-MS/MS and GC-MS applications, correcting for instrument variability and sample preparation losses.
• Biomedical Research: Used in studies investigating cholesterol biosynthesis, metabolism, and related disorders such as atherosclerosis and Niemann-Pick disease.
• Pharmaceutical Development: Supports pharmacokinetic (PK) and pharmacodynamic (PD) studies for drug candidates targeting lipid pathways, ensuring accurate bioanalytical data.
• Clinical Diagnostics: Enables the development and validation of highly accurate diagnostic assays for measuring sterol levels in patient serum or plasma samples.
• Metabolomics and Lipidomics: A key tool in high-throughput profiling of sterols and other lipids, facilitating biomarker discovery and systems biology research.
• Quality Control in Reference Material Production: Serves as a calibrant or reference compound in the production of certified reference materials (CRMs) for clinical and research laboratories.

Basic Information

Product Name	6α-Hydroxy-5α-Cholestane-D7
CAS No.	1246302-83-9
Molecular Formula	C₂₇H₄₁D₇O
Molecular Weight	~395.7 g/mol
Synonyms	6α-Hydroxy-5α-cholestane-d7; (3β,5α,6α)-Cholestane-3,6-diol-d7; 5α-Cholestane-3β,6α-diol-d7; Deuterated 6α-Hydroxy-5α-cholestane; 6α-Hydroxycholestane-d7; d7-6α-Hydroxy-5α-cholestane; Cholestane-3β,6α-diol-d7; Internal Standard for Cholesterol Metabolism
EINECS	Contact for details

Quality Control

Every batch of 6α-Hydroxy-5α-Cholestane-D7 is manufactured and tested under strict quality management systems. We guarantee high chemical purity and isotopic enrichment, verified by advanced analytical techniques including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, isotopic distribution, and analytical results is provided with each shipment to ensure traceability and compliance with your research or quality protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a cool, dry place. This product is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere after each use to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D7)	≥ 98.0 atom % D
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.