Description

Tamra-Peg3-Azide is a fluorescent labeling reagent featuring a tetramethylrhodamine (TAMRA) dye conjugated to a triethylene glycol (PEG3) spacer terminated with an azide functional group. This compound is engineered for high-efficiency bioorthogonal conjugation via copper-catalyzed or strain-promoted azide-alkyne cycloaddition (CuAAC/SPAAC) click chemistry. It is essential for researchers and developers in bioconjugation, chemical biology, and diagnostic assay development who require precise, stable fluorescent tagging of biomolecules such as proteins, antibodies, and oligonucleotides.

Application

• Bioconjugation and Protein Labeling: Site-specific fluorescent labeling of antibodies, enzymes, and other proteins for imaging and detection.
• Chemical Biology Probes: Synthesis of activity-based probes (ABPs) or pull-down reagents to study protein function and interactions.
• Diagnostic Assay Development: Conjugation to oligonucleotides or other biomolecules for use in fluorescence in situ hybridization (FISH), microarray, or lateral flow assays.
• Cell Imaging and Tracking: Labeling of cell surface glycans or other targets for live-cell or fixed-cell fluorescence microscopy.
• Material Science Functionalization: Modification of nanoparticles, polymers, or surfaces with fluorescent properties for biosensing applications.
• Drug Delivery Research: Conjugation to drug carriers or linkers to visualize targeting and uptake mechanisms.

Basic Information

Product Name	Tamra-Peg3-Azide
CAS No.	1228100-59-1
Molecular Formula	C35H41N7O8
Molecular Weight	687.75 g/mol
Synonyms	5-TAMRA-PEG3-Azide; 5-Carboxytetramethylrhodamine-PEG3-Azide; TAMRA triethylene glycol azide; 6-((2-(2-(2-Azidoethoxy)ethoxy)ethoxy)ethylamino)-2-(3,6-bis(dimethylamino)xanthylium-9-yl)benzoate; Xanthylium, 9-[2-(carboxy-4-[(2,5-dioxo-1-pyrrolidinyl)oxy]-4-oxobutyl)phenyl]-3,6-bis(dimethylamino)-, inner salt, 2,5-dioxo-1-pyrrolidinyl ester (related precursor); TAMRA-N3; Rhodamine B Azide PEG3 derivative
EINECS	Contact for details

Quality Control

Our Tamra-Peg3-Azide is manufactured under strict quality protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. We provide a Certificate of Analysis (COA) with each shipment, detailing batch-specific results to ensure consistency and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.