Description

5-Bromo-3-Indolyl-β-D-Galactopyranoside is a chromogenic substrate specifically designed for the detection of β-galactosidase activity. This compound matters for its critical role in molecular biology and diagnostic applications, where it serves as a precise and reliable indicator. It is primarily needed by researchers and manufacturers in the fields of biotechnology, pharmaceutical development, and clinical diagnostics for applications such as gene expression analysis and microbial identification.

Application

• Molecular Biology Research: As a key substrate in blue-white screening for recombinant clone selection in plasmid vectors containing the lacZ gene.
• Enzyme Assays: For the quantitative and qualitative measurement of β-galactosidase enzyme activity in cell lysates and purified samples.
• Microbiology & Diagnostics: Used in culture media for the identification of microorganisms, such as E. coli, based on β-galactosidase production.
• Reporter Gene Studies: Employed in histochemical staining to visualize the spatial and temporal expression of genes fused to the lacZ reporter in tissues and cells.
• High-Throughput Screening (HTS): Utilized in biochemical assays for drug discovery to identify modulators of β-galactosidase or related pathways.
• Biochemical Reagent Manufacturing: Serves as a critical raw material for producing diagnostic kits and specialized research reagents.

Basic Information

Item	Details
Product Name	5-Bromo-3-Indolyl-β-D-Galactopyranoside
CAS No.	1226578-88-6
Molecular Formula	C14H16BrNO6
Molecular Weight	374.18 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal); 5-Bromo-3-indolyl β-D-galactoside; X-Gal; BCIG; 5-Bromo-4-chloro-1H-indol-3-yl β-D-galactopyranoside; Galactoside indoxyl bromochloro; β-Galactosidase substrate X-Gal
EINECS	Contact for details

Quality Control

Our 5-Bromo-3-Indolyl-β-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and functional testing in enzymatic assays. Certificates of Analysis (COA) detailing all specifications are available upon request, supporting compliance with research and diagnostic-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Functional Activity	Conforms to reference standard in β-galactosidase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.