Description

Tamra-Peg4-Alkyne is a high-purity, functionalized chemical reagent designed for advanced bioconjugation and labeling applications. Its core value lies in the combination of a bright, photostable TAMRA fluorophore, a flexible tetraethylene glycol (PEG4) spacer, and a reactive alkyne handle, enabling efficient, site-specific labeling via copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. This compound is essential for researchers and manufacturers in pharmaceutical development, proteomics, bioimaging, and diagnostic assay creation who require precise and stable fluorescent tagging of biomolecules.

Application

• Fluorescent Labeling of Biomolecules: Site-specific conjugation to azide-modified proteins, antibodies, oligonucleotides, and small molecules for tracking and detection.
• Live-Cell and In-Vivo Imaging Probes: Development of fluorescent probes for cellular uptake studies, receptor targeting, and biodistribution analysis due to TAMRA's favorable optical properties.
• Proteomics and Biomarker Discovery: Tagging of target proteins or peptides in activity-based protein profiling (ABPP) and pull-down assays.
• Diagnostic Assay Development: Conjugation to detection antibodies or antigens in ELISA, lateral flow assays, and other immunoassays.
• Polymer and Materials Science: Functionalization of azide-containing polymers, surfaces, or nanoparticles to introduce a fluorescent label for characterization.
• Chemical Biology Tool Development: Creation of novel chemical probes for studying biological pathways and interactions.

Basic Information

Product Name	Tamra-Peg4-Alkyne
CAS No.	1225057-68-0
Molecular Formula	C₄₈H₆₀N₄O₁₂
Molecular Weight	873.0 g/mol
Synonyms	5-TAMRA-PEG4-Alkyne; TAMRA tetraethylene glycol alkyne; 5-Carboxytetramethylrhodamine-PEG4-propargylamide; 6-TAMRA-PEG4-Alkyne; Tetramethylrhodamine-PEG4-Alkyne; 5/6-TAMRA-PEG4-Propargylamide; TAMRA-PEG4-Propargyl Amide; N-(Propargyl)-TAMRA-PEG4-amide
EINECS	Contact for details

Quality Control

Our Tamra-Peg4-Alkyne is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry (MS) for identity confirmation, and NMR for structural verification. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption (λmax)	~554 nm
Emission (λmax)	~580 nm
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.