Description

5-Bromo-6-Chloro-3-Indolyl-α-L-Fucopyranoside is a specialized chromogenic substrate used in enzymatic detection and histochemical staining applications. Its core value lies in its ability to produce an insoluble, colored precipitate upon enzymatic cleavage, making it an essential tool for visualizing specific biological activity. This compound is primarily needed by researchers and manufacturers in the life sciences, particularly for applications in molecular biology, diagnostic assay development, and carbohydrate research.

Application

• Enzymatic Substrate for Glycosidases: Specifically used as a substrate for α-L-fucosidase in biochemical assays and research.
• Histochemical Staining: Employed in tissue staining to localize and visualize α-L-fucosidase activity in cells and biological samples.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for detecting enzyme deficiencies or specific biomarkers.
• Microbiology Research: Used to study bacterial glycosidase activity and for the identification of microorganisms.
• Biochemical Pathway Analysis: Aids in the study of fucose metabolism and related glycosylation pathways in cell biology.
• Quality Control in Bioprocessing: Utilized to monitor enzyme activity and purity in the production of therapeutic proteins and other biologics.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indolyl-α-L-Fucopyranoside
CAS No.	1222705-28-3
Molecular Formula	C14H15BrClNO5
Molecular Weight	400.63 g/mol
Synonyms	5-Bromo-6-chloro-3-indolyl α-L-fucopyranoside; X-α-L-Fuc; 5-Br-6-Cl-3-indoxyl-α-L-fucopyranoside; BCIP-α-L-Fuc; α-L-Fucosidase Substrate; Chromogenic α-L-Fucoside; 1222705-28-3; 3-Indolyl-α-L-fucopyranoside, 5-bromo-6-chloro- derivative
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indolyl-α-L-Fucopyranoside is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) documenting purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.