Description

Methylphenidate-D9 HCl (Racemic Mixture of Erythro and Threo Isomers) is a stable isotope-labeled analog of the central nervous system stimulant methylphenidate, where nine hydrogen atoms are replaced by deuterium. This compound is essential as a high-purity internal standard for the accurate quantification and pharmacokinetic profiling of methylphenidate in complex biological matrices using advanced analytical techniques. It is a critical tool for pharmaceutical R&D, forensic toxicology, and clinical laboratories engaged in drug metabolism and bioequivalence studies.

Application

• Internal Standard for LC-MS/MS: Primary application as a deuterated internal standard for the precise and sensitive quantification of methylphenidate and its metabolites in plasma, serum, and urine via liquid chromatography-tandem mass spectrometry (LC-MS/MS).
• Pharmacokinetic & Metabolism Studies: Enables accurate determination of absorption, distribution, metabolism, and excretion (ADME) profiles in preclinical and clinical research.
• Forensic & Clinical Toxicology: Used in confirmatory testing and monitoring of methylphenidate (Ritalin®) use, abuse, and compliance in forensic and workplace drug testing programs.
• Bioanalytical Method Development & Validation: Serves as a critical reference material for developing, validating, and ensuring the regulatory compliance (e.g., FDA, EMA guidelines) of analytical methods.
• Pharmaceutical Quality Control: Supports impurity profiling and stability testing of methylphenidate-based drug products.
• Isotope Dilution Analysis: Fundamental for high-accuracy measurements where the deuterated analog compensates for matrix effects and instrument variability.

Basic Information

Product Name	Methylphenidate-D9 HCl (Racemic Mixture of Erythro and Threo Isomers)
CAS No.	1219804-02-0
Molecular Formula	C₁₄H₁₀D₉NO₂•HCl
Molecular Weight	~ 284.8 g/mol (for free base C₁₄H₁₀D₉NO₂)
Synonyms	Methylphenidate-d9 Hydrochloride; dl-Methylphenidate-d9 HCl; (±)-Methylphenidate-d9 Hydrochloride; α-Phenyl-2-piperidineacetic acid methyl ester-d9 hydrochloride; Ritalin-d9 HCl; MPH-d9 HCl; Methyl α-phenyl-2-piperidineacetate-d9 hydrochloride; Deuterated Methylphenidate HCl (D9)
EINECS	Contact for details

Quality Control

Our Methylphenidate-D9 HCl is manufactured under strict quality management systems to ensure the highest standards of isotopic purity and chemical integrity. Each batch is characterized by advanced spectroscopic and chromatographic methods, including NMR, HPLC, and LC-MS, to confirm identity, chemical purity, and isotopic enrichment (typically ≥98 atom % D). A comprehensive Certificate of Analysis (COA) detailing all specifications and test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (LC-MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (Atom % D)	≥ 98%
Chemical Purity (by NMR)	≥ 95%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.