Description

5-Hydroxyindole-4,6,7-D3-3-Acetic--D2 Acid (5-Hydroxyindole-4,6,7-D3-3-Acetic-2,2-D2 Acid) is a high-purity, deuterium-labeled derivative of 5-hydroxyindole-3-acetic acid, a key metabolite of serotonin. This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy and reproducibility in analytical workflows. It is critically important for researchers and quality control laboratories in the pharmaceutical, clinical diagnostics, and neurochemical research sectors who require definitive measurement of serotonin pathway metabolites.

Application

• Internal Standard for LC-MS/MS: Primary application as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for the precise measurement of 5-HIAA and related indole metabolites.
• Biomarker Research & Validation: Used in the development and validation of clinical assays for serotonin-related disorders, carcinoid syndrome, and neurological research.
• Drug Metabolism & Pharmacokinetics (DMPK): Enables accurate tracking of drug-induced changes in serotonin metabolism during preclinical and clinical studies.
• Clinical Diagnostics: Critical for ensuring the accuracy of commercial diagnostic kits that measure urinary or plasma 5-HIAA levels.
• Forensic Toxicology: Supports definitive analysis of serotonin and its metabolites in forensic investigations.
• Reference Material Production: Serves as a certified component in the preparation of accredited reference materials (CRMs) for laboratory proficiency testing.

Basic Information

Product Name	5-Hydroxyindole-4,6,7-D3-3-Acetic--D2 Acid / 5-Hydroxyindole-4,6,7-D3-3-Acetic-2,2-D2 Acid
CAS No.	1219802-93-3
Molecular Formula	C10H4D5NO3
Molecular Weight	~195.21 g/mol (for deuterated form)
Synonyms	5-HIAA-d5; 5-Hydroxyindole-3-acetic acid-d5; 5-Hydroxy-3-indoleacetic acid-d5; [2,2,4,6,7-D5]-5-Hydroxyindole-3-acetic acid; Serotonin metabolite-d5; Deuterated 5-HIAA; 5-Hydroxy-1H-indole-3-acetic-2,2,4,6,7-d5 acid; 5-HIAA Internal Standard
EINECS	Contact for details

Quality Control

Every batch of our deuterated 5-Hydroxyindole-4,6,7-D3-3-Acetic-2,2-D2 Acid is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity as verified by advanced spectroscopic and chromatographic methods, including NMR, HPLC, and MS. Certificates of Analysis (COA) are provided with each shipment, detailing batch-specific data on purity, isotopic enrichment, and solvent content to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to expected mass spectrum
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.