Description

n-(4-Hydroxyphenyl-2,3,5,6-D4)Acetamide-2,2,2-D3 is a high-purity, deuterium-labeled analog of acetaminophen (paracetamol), specifically designed for use as an internal standard in analytical chemistry. This compound is critical for ensuring the accuracy and reliability of quantitative mass spectrometry (MS) and nuclear magnetic resonance (NMR) analyses by correcting for variations in sample preparation and instrument response. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, forensic, and clinical diagnostics industries who require precise and traceable measurements of acetaminophen and its metabolites.

Application

• Internal Standard for LC-MS/MS: Primary application as a stable, non-interfering internal standard for the precise quantification of acetaminophen in biological matrices (plasma, serum, urine) during pharmacokinetic and bioequivalence studies.
• Metabolite Tracing: Used in drug metabolism and disposition (DMPK) studies to track and quantify the formation of deuterated metabolites, providing insights into metabolic pathways.
• Pharmaceutical Quality Control: Enables accurate assay and impurity profiling of acetaminophen in active pharmaceutical ingredients (APIs) and finished dosage forms, ensuring compliance with pharmacopeial standards (USP, EP).
• Forensic and Toxicology Analysis: Supports the accurate determination of acetaminophen levels in cases of overdose or poisoning in forensic toxicology laboratories.
• Clinical Research: Facilitates reliable measurement of drug concentrations in patient samples for therapeutic drug monitoring (TDM) and clinical trial support.
• Method Development and Validation: Serves as a critical component in developing, optimizing, and validating robust analytical methods for regulatory submission.

Basic Information

Item	Details
Product Name	n-(4-Hydroxyphenyl-2,3,5,6-D4)Acetamide-2,2,2-D3
CAS No.	1219798-53-4
Molecular Formula	C8D7H2NO2
Molecular Weight	155.20 g/mol
Synonyms	Acetaminophen-d7; Paracetamol-d7; 4'-Hydroxyacetanilide-d7; APAP-d7; N-(4-Hydroxyphenyl-d4)acetamide-2,2,2-d3; N-Acetyl-4-aminophenol-d7; 4-Acetamidophenol-d7; Deuterated Acetaminophen
EINECS	Contact for details

Quality Control

Every batch of n-(4-Hydroxyphenyl-2,3,5,6-D4)Acetamide-2,2,2-D3 is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure exceptional isotopic purity and chemical identity. Our quality assurance protocols are designed to meet the stringent requirements of analytical reference standard applications. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing results for isotopic purity (by 1H NMR or MS), chemical purity (by HPLC), and other critical parameters.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity	≥ 98 atom % D
Chemical Purity	≥ 95% (by HPLC)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.