Description

2-Demethylcolchicine-D3 is a deuterated analog of a key colchicine derivative, serving as a critical stable isotope-labeled internal standard for precise analytical quantification. This high-purity reference material is essential for ensuring accuracy and reliability in method development and validation within regulated analytical workflows. It is primarily required by pharmaceutical research laboratories, contract research organizations (CROs), and analytical testing facilities engaged in pharmacokinetic studies, metabolic profiling, and quality control of colchicine-related compounds.

Application

• Internal Standard for LC-MS/MS: Primary application as a deuterium-labeled internal standard for the accurate quantification of colchicine and its metabolites in biological matrices (e.g., plasma, serum, urine) using liquid chromatography-tandem mass spectrometry.
• Pharmacokinetic and Bioanalytical Research: Enables precise measurement of drug concentration-time profiles, supporting ADME (Absorption, Distribution, Metabolism, Excretion) studies in preclinical and clinical research.
• Method Development and Validation: Critical for developing robust, sensitive, and specific analytical methods compliant with regulatory guidelines (e.g., FDA, EMA) for bioanalysis.
• Metabolite Identification and Profiling: Aids in the identification and quantification of demethylated metabolites of colchicine in metabolic stability assays.
• Quality Control Reference Standard: Used as a certified reference material (CRM) to calibrate instruments and verify assay performance in quality control laboratories.
• Forensic and Doping Control Analysis: Supports accurate detection and quantification of colchicine in forensic toxicology and anti-doping programs.

Basic Information

Product Name	2-Demethylcolchicine-D3
CAS No.	1217630-52-8
Molecular Formula	C21H20D3NO6
Molecular Weight	~383.42 g/mol
Synonyms	N-Deacetyl-N-(2-demethyl)colchicine-D3; Colchicine-2-demethyl-D3; 2-Demethylcolchicine deuterated; (S)-N-(5,6,7,9-Tetrahydro-1,2,3,10-tetramethoxy-9-oxobenzo[a]heptalen-7-yl)acetamide-2-demethyl-D3; 2-O-Demethylcolchicine-D3; Colchiceine-2-demethyl-D3 methyl ether; Stable Isotope Labeled 2-Demethylcolchicine; [2H3]-2-Demethylcolchicine
EINECS	Contact for details

Quality Control

Every batch of 2-Demethylcolchicine-D3 is manufactured and analyzed under strict quality management systems. We provide comprehensive analytical data to support its use as a high-grade reference standard. Our quality commitment includes rigorous testing for isotopic purity, chemical purity, and structural confirmation. Certificates of Analysis (COA) are supplied with each shipment, detailing batch-specific results from NMR (1H, 13C), HPLC, and LC-MS analyses.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D3)	≥ 98.0 atom % D
Chemical Purity (by NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.