Description

Colchicine-D3 is a stable isotope-labeled analog of the natural alkaloid colchicine, where three hydrogen atoms are replaced by deuterium (CAS NO 1217625-62-1). This compound serves as a critical internal standard for the precise and accurate quantification of colchicine in complex biological matrices using advanced analytical techniques like LC-MS/MS. It is an essential tool for pharmaceutical research and development, clinical diagnostics, and forensic toxicology laboratories requiring high-fidelity analytical data.

Application

• Internal Standard for Bioanalysis: Primary use as a deuterated internal standard in Liquid Chromatography-Mass Spectrometry (LC-MS/MS) for the quantification of colchicine in plasma, serum, and urine.
• Pharmacokinetic and Metabolism Studies: Enables accurate tracking of colchicine absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research.
• Therapeutic Drug Monitoring (TDM): Supports the development and validation of robust assays to monitor colchicine levels in patients, ensuring therapeutic efficacy and minimizing toxicity risks.
• Forensic and Clinical Toxicology: Used in confirmatory testing for colchicine poisoning or overdose investigations, providing high specificity and reliability.
• Method Development and Validation: Critical for developing new analytical methods, ensuring compliance with regulatory guidelines (e.g., FDA, EMA) for bioanalytical method validation.
• Reference Material for Quality Control: Serves as a certified reference material in quality control laboratories to verify the accuracy of colchicine assays in pharmaceutical formulations.

Basic Information

Product Name	Colchicine-D3
CAS No.	1217625-62-1
Molecular Formula	C22H22D3NO6
Molecular Weight	402.46 g/mol (approximate)
Synonyms	Deuterated Colchicine (d3); Colchicine-d3; (S)-N-((7S)-1,2,3,10-Tetramethoxy-9-oxo-6,7-dihydro-5H-benzo[a]heptalen-7-yl)acetamide-d3; Colchicine (methyl-d3); Colchicine Internal Standard; Stable Isotope-Labeled Colchicine; Colchicine-D3 for LC-MS; Colchicine (Trideuteriomethyl-)
EINECS	Contact for details

Quality Control

Our Colchicine-D3 is manufactured under strict quality control protocols to ensure the highest standards of isotopic purity and chemical integrity, suitable for sensitive analytical applications. Each batch is characterized by advanced spectroscopic methods (NMR, HRMS) and undergoes rigorous purity analysis by HPLC to meet exacting research requirements. A comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically ≥98% D), chemical purity (≥95% by HPLC), and analytical data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Isotopic Purity (D-content)	≥ 98 atom % D
Chemical Purity (by HPLC)	≥ 95%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.