Description

Isosorbide-13C6 2-Nitrate is a stable isotope-labeled derivative of isosorbide mononitrate, where six carbon atoms are replaced with the 13C isotope. This compound is critically important as an internal standard for the precise quantification of isosorbide mononitrate and related metabolites in complex biological matrices using advanced analytical techniques. It is an essential tool for researchers and quality control laboratories in the pharmaceutical and life sciences sectors, enabling accurate pharmacokinetic studies, bioequivalence assessments, and method development.

Application

• Internal Standard for LC-MS/MS Analysis: Primary use as a quantitative internal standard in liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the accurate measurement of isosorbide mononitrate in plasma, serum, and other biological samples.
• Pharmacokinetic and Bioanalytical Research: Enables precise tracking of drug absorption, distribution, metabolism, and excretion (ADME) in clinical and preclinical studies for cardiovascular drug development.
• Metabolite Profiling and Identification: Aids in the identification and quantification of nitrate ester metabolites in metabolic pathway studies.
• Method Development and Validation: Serves as a critical reference material for developing, optimizing, and validating robust analytical methods in compliance with GLP and GMP guidelines.
• Quality Control in Pharmaceutical Manufacturing: Used in in-process testing and final product release assays to ensure the potency and purity of isosorbide mononitrate-based drug formulations.
• Stable Isotope Dilution Assays (SIDA): Fundamental component in high-accuracy SIDA protocols to minimize matrix effects and ionization variability in mass spectrometry.

Basic Information

Item	Details
Product Name	Isosorbide-13C6 2-Nitrate
CAS No.	1217604-00-6
Molecular Formula	C613C6H9NO6
Molecular Weight	~207.1 g/mol (for 13C6 labeled form)
Synonyms	1,4:3,6-Dianhydro-D-glucitol-13C6 2-Nitrate; Isosorbide-13C6 Mononitrate; 1,4:3,6-Dianhydro-D-sorbitol-13C6 2-Nitrate; D-Isosorbide-13C6 2-Mononitrate; ISMN-13C6; Isotopically Labeled Isosorbide Mononitrate; (3R,3aR,6S,6aR)-Hexahydrofuro[3,2-b]furan-3,6-diyl-13C6 2-Nitrate
EINECS	Contact for details

Quality Control

Every batch of Isosorbide-13C6 2-Nitrate is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity to meet the exacting demands of quantitative bioanalysis. Comprehensive testing includes assay by HPLC, confirmation of isotopic enrichment by MS, and control of related substances. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting batch-specific results for identity, purity, and isotopic abundance.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the certificate of analysis. Keep the container in a dry, well-ventilated place. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (13C6)	≥ 99 atom % 13C
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.