Description

trans-3'-Hydroxycotinine-o-(4-Deoxy-4,5-Didehydro)--D-Glucuronide, Methyl Ester is a high-purity, chemically defined metabolite reference standard. This compound is critical for analytical research and method development, providing a reliable benchmark for accurate quantification and identification. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and diagnostic manufacturers specializing in nicotine metabolism studies and toxicology screening.

Application

• Reference Standard for Bioanalytical Assays: Used as a primary standard in LC-MS/MS and HPLC methods for quantifying nicotine metabolites in biological matrices like plasma, urine, and saliva.
• Pharmacokinetic and Metabolic Research: Essential for studying the metabolic pathways, clearance rates, and bioavailability of cotinine and related alkaloids in clinical and preclinical studies.
• Diagnostic Kit Development: Serves as a key calibrant and quality control material in the manufacturing of immunoassays and other diagnostic tests for tobacco exposure monitoring.
• Forensic Toxicology: Employed as a certified reference material for the precise and legally defensible analysis of nicotine consumption in forensic applications.
• Pharmaceutical Impurity Profiling: Used to identify and quantify related substances and degradation products in nicotine replacement therapy (NRT) products for quality control.
• Academic and Government Research: Supports public health studies investigating smoking behavior, addiction mechanisms, and the health impacts of tobacco use.

Basic Information

Product Name	trans-3'-Hydroxycotinine-o-(4-Deoxy-4,5-Didehydro)--D-Glucuronide, Methyl Ester
CAS No.	1217540-34-5
Molecular Formula	C₁₈H₂₄N₂O₈
Molecular Weight	396.39 g/mol
Synonyms	trans-3'-Hydroxycotinine O-Glucuronide Methyl Ester; (3'R,5S)-3'-Hydroxycotinine 4,5-Didehydro-O-glucuronide Methyl Ester; 4,5-Didehydro-3'-hydroxycotinine O-β-D-glucopyranuronoside Methyl Ester; Hydroxycotinine Glucuronide Methyl Ester; trans-3'-OH-Cotinine Glucuronide Methyl Ester; Nicotine Metabolite Reference Standard; Cotinine Phase II Metabolite Derivative
EINECS	Contact for details

Quality Control

Our trans-3'-Hydroxycotinine-o-(4-Deoxy-4,5-Didehydro)--D-Glucuronide, Methyl Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC purity assay, identity confirmation by spectroscopic methods (NMR, MS), and residual solvent analysis to ensure it meets the stringent requirements for reference standard applications. A detailed Certificate of Analysis (COA) with batch-specific data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing at -20°C under inert atmosphere to ensure maximum stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference retention time
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.