Description

n-(9-Fluorenylmethoxycarbonyl)-L-Proline-13C5,15N, L-Proline-13C5,15N, n-Fmoc Derivative is a high-purity, isotopically labeled derivative of the amino acid proline, specifically designed for advanced research and development applications. This compound is essential for scientists requiring precise metabolic tracing and structural elucidation in complex biochemical systems. It is primarily utilized by researchers in pharmaceutical development, proteomics, and metabolic studies where high-fidelity isotopic labeling is critical for accurate data.

Application

• Stable Isotope Labeling in Proteomics: Used as a building block for synthesizing isotopically labeled peptides and proteins for mass spectrometry-based quantitative analysis.
• Metabolic Pathway Tracing: Enables the study of proline metabolism, collagen synthesis, and other biochemical pathways in living systems using NMR or LC-MS.
• Pharmaceutical Research & Development: Serves as a key intermediate in the synthesis of isotopically labeled drug candidates for pharmacokinetic (PK) and absorption, distribution, metabolism, and excretion (ADME) studies.
• Biomolecular NMR Spectroscopy: Provides a source of 13C and 15N labels for structural and dynamic studies of proteins and peptides.
• Internal Standard for Quantitative Analysis: Acts as a high-purity internal standard in analytical methods like HPLC-MS/MS for the accurate quantification of proline and related compounds.
• Peptide Synthesis (SPPS): The Fmoc-protected form is used in solid-phase peptide synthesis for the controlled incorporation of labeled proline residues.

Basic Information

Product Name	n-(9-Fluorenylmethoxycarbonyl)-L-Proline-13C5,15N, L-Proline-13C5,15N, n-Fmoc Derivative
CAS No.	1217452-48-6
Molecular Formula	C20H17[13C]5[15N]O4
Molecular Weight	~386.36 g/mol (based on labeled atoms)
Synonyms	Fmoc-L-proline-13C5,15N; (S)-1-((((9H-Fluoren-9-yl)methoxy)carbonyl)-13C5,15N)pyrrolidine-2-carboxylic acid; N-α-Fmoc-L-proline-13C5,15N; Fmoc-Pro-13C5,15N-OH; N-(9-Fluorenylmethoxycarbonyl)-L-proline-13C5,15N; Isotopically labeled Fmoc-proline; 13C5,15N-Fmoc-Proline; Fmoc-protected L-proline-13C5,15N
EINECS	Contact for details

Quality Control

Our n-(9-Fluorenylmethoxycarbonyl)-L-Proline-13C5,15N is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment, chemical purity, and identity confirmation. We adhere to relevant industry guidelines for the production of stable isotope-labeled compounds to ensure reliable and reproducible performance in your experiments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This compound is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (13C)	≥ 99 atom % 13C
Isotopic Enrichment (15N)	≥ 99 atom % 15N
Chemical Purity (NMR)	Conforms
Specific Rotation	Contact for details
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.