Description

7-((9-Fluorenylmethoxycarbonyl)Cyclohexylalanyllysyl)Amino-4-Methylcoumarin Trifluoroacetate Salt is a specialized, high-purity synthetic peptide derivative designed for advanced biochemical research and pharmaceutical development. This compound is critical for researchers requiring a precisely engineered substrate or intermediate with a fluorescent coumarin reporter group and Fmoc-protected amino acids. It is primarily utilized by scientists in proteomics, enzymology, and medicinal chemistry for developing sensitive assays and investigating enzyme kinetics and inhibition.

Application

• Protease & Enzyme Substrate: Acts as a specialized fluorogenic substrate for studying the activity and specificity of proteolytic enzymes, including caspases and other serine or cysteine proteases.
• Biochemical Assay Development: Serves as a key component in the design and optimization of high-throughput screening (HTS) assays for drug discovery pipelines.
• Peptide Synthesis Intermediate: Used as a building block in solid-phase peptide synthesis (SPPS) for constructing complex peptides with specific fluorescent labels.
• Cell Biology Research: Employed in live-cell imaging and intracellular activity profiling to monitor enzyme function in real-time.
• Diagnostic Reagent Formulation: A potential component in the development of diagnostic kits for detecting specific enzymatic activities linked to disease states.
• Academic & Pharmaceutical R&D: Essential for fundamental research in universities and for target validation studies in pharmaceutical companies.

Basic Information

Item	Detail
Product Name	7-((9-Fluorenylmethoxycarbonyl)Cyclohexylalanyllysyl)Amino-4-Methylcoumarin Trifluoroacetate Salt
CAS No.	1217448-19-5
Molecular Formula	C45H53F3N6O9
Molecular Weight	878.94 g/mol
Synonyms	Fmoc-Cha-Lys(AMC)-OH•TFA; Ac-AMC Substrate Derivative; 7-Amino-4-methylcoumarin (AMC) Peptide Conjugate; Fmoc-Cyclohexylalanine-Lysine(AMC)-Trifluoroacetate; Fluorogenic Peptide Substrate; Caspase Substrate Analog; (9H-Fluoren-9-yl)methyl N-[(2S)-1-[[(2S)-6-amino-1-[(7-amino-4-methyl-2-oxo-2H-chromen-8-yl)amino]-1-oxohexan-2-yl]amino]-3-cyclohexyl-1-oxopropan-2-yl]carbamate trifluoroacetate
EINECS	Contact for details

Quality Control

Our production of this advanced biochemical adheres to stringent quality protocols suitable for research and development. Every batch is characterized using advanced analytical techniques to ensure identity, purity, and consistency. A comprehensive Certificate of Analysis (COA) is provided, detailing results for assay, impurity profile, and spectroscopic identification. We support compliance with ISO 9001 quality management principles, and our processes are designed to meet the exacting standards required for pharmaceutical R&D applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular weight
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 3.0%
Residual Solvents (GC)	Meets ICH guidelines
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.