Description

4,6-Dihydroxypyrazolo[3,4-D]Pyrimidine-13C,15N2 is a stable isotope-labeled analog of a key pyrazolopyrimidine heterocyclic scaffold, essential for advanced analytical and research applications. This compound is specifically designed for use as an internal standard in mass spectrometry, enabling precise quantification and metabolic pathway tracing in complex biological matrices. It is a critical material for researchers and developers in pharmaceutical R&D, particularly for drug metabolism and pharmacokinetics (DMPK) studies, bioanalytical method development, and the synthesis of labeled active pharmaceutical ingredients (APIs).

Application

• Internal Standard for LC-MS/MS: Primary application as a quantitative internal standard in liquid chromatography-tandem mass spectrometry for bioanalytical assays.
• Drug Metabolism Studies (DMPK): Used to trace and quantify metabolites of pyrazolopyrimidine-based drug candidates in preclinical and clinical research.
• Isotope Dilution Mass Spectrometry (IDMS): Enables high-accuracy quantification in pharmacokinetic and toxicokinetic studies.
• Synthesis of Labeled APIs: Serves as a building block for the synthesis of carbon-13 and nitrogen-15 labeled pharmaceutical compounds.
• Biomedical Research: Tool compound for investigating the role of purine analog pathways in cellular processes.
• Quality Control & Method Validation: Critical for validating analytical methods in GLP/GMP-compliant laboratories to ensure accuracy and reproducibility.

Basic Information

Product Name	4,6-Dihydroxypyrazolo[3,4-D]Pyrimidine-13C,15N2
CAS No.	1217036-71-9
Molecular Formula	C413C H4 N215N2 O2
Molecular Weight	~157.06 g/mol (for labeled form)
Synonyms	4,6-Dihydroxypyrazolo[3,4-d]pyrimidine-13C,15N2; 1H-Pyrazolo[3,4-d]pyrimidine-4,6-diol-13C,15N2; Allopurinol Impurity/Intermediate-13C,15N2; Isotopically Labeled Pyrazolopyrimidine; [13C,15N2]-4,6-Dihydroxy-1H-pyrazolo[3,4-d]pyrimidine; 4,6-Dihydroxy-1H-pyrazolo[3,4-d]pyrimidine (13C,15N2); Labeled Allopurinol Related Compound
EINECS	Contact for details

Quality Control

Our 4,6-Dihydroxypyrazolo[3,4-D]Pyrimidine-13C,15N2 is manufactured under strict quality systems to ensure the highest isotopic and chemical purity for sensitive analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (Atom % 13C and 15N), chemical purity by HPLC, and identity confirmation by NMR and MS. We adhere to relevant industry standards for stable isotope-labeled compounds, ensuring reliability for GLP and research use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (13C)	≥ 99 atom %
Isotopic Enrichment (15N)	≥ 98 atom %
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.