Description

Amiloride-15N3 Hydrochloride is a stable isotope-labeled analog of the potassium-sparing diuretic amiloride, where three nitrogen atoms are replaced with the 15N isotope. This compound is essential for use as an internal standard in quantitative bioanalytical methods, ensuring high accuracy and precision in mass spectrometry-based assays. It is primarily required by pharmaceutical research and development laboratories, clinical research organizations (CROs), and metabolomics facilities for pharmacokinetic studies, drug metabolism research, and the development of validated LC-MS/MS methods.

Application

• Internal Standard for LC-MS/MS: Serves as a critical quantitative reference standard in Liquid Chromatography-Tandem Mass Spectrometry for the analysis of amiloride in biological matrices like plasma, serum, and urine.
• Pharmacokinetic and Bioavailability Studies: Enables precise tracking and quantification of amiloride absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research.
• Drug Metabolism Research: Used in metabolite identification and profiling studies to understand the biotransformation pathways of amiloride.
• Clinical Research and Therapeutic Drug Monitoring (TDM): Supports the development and validation of robust assays for monitoring amiloride concentrations in patient samples.
• Forensic and Anti-Doping Analysis: Provides a definitive isotopic standard for confirmatory testing in forensic toxicology and sports drug testing programs.
• Metabolomics and Biomarker Discovery: Facilitates accurate quantification in studies investigating the role of amiloride or related pathways in disease states.

Basic Information

Product Name	Amiloride-15N3 Hydrochloride
CAS No.	1216796-18-7
Molecular Formula	C6H8Cl215N3O·HCl
Molecular Weight	~ 269.5 g/mol (for 15N3 labeled form)
Synonyms	Amiloride-15N3 HCl; 3,5-Diamino-N-(aminoiminomethyl)-6-chloropyrazinecarboxamide-15N3 Hydrochloride; Amiloride (N-amidino-3,5-diamino-6-chloropyrazinecarboxamide)-15N3 Hydrochloride; Amiloride D5 (common vendor listing); [15N3]-Amiloride Hydrochloride; Stable Isotope Labeled Amiloride HCl; Amiloride Internal Standard
EINECS	Contact for details

Quality Control

Our Amiloride-15N3 Hydrochloride is manufactured under strict quality control protocols to ensure the highest isotopic and chemical purity for demanding analytical applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (typically >98 atom % 15N), chemical purity by HPLC, and confirmation of structure by NMR and MS. We adhere to relevant industry standards for reference materials to ensure data integrity and reproducibility in your research.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % 15N
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.