Description

n-Acetyl Sulfamethoxazole-D4 is a deuterium-labeled internal standard of the acetylated metabolite of the antibiotic sulfamethoxazole. This high-purity compound is essential for ensuring the accuracy and reliability of quantitative mass spectrometry analyses in pharmacokinetic and bioequivalence studies. It is primarily used by analytical laboratories and pharmaceutical R&D departments engaged in drug metabolism and residue testing.

Application

• Quantitative Internal Standard for LC-MS/MS: Critical for the precise quantification of sulfamethoxazole and its metabolites in biological matrices (plasma, urine, tissue).
• Pharmacokinetic and Bioanalytical Research: Enables accurate tracking of drug absorption, distribution, metabolism, and excretion (ADME) studies.
• Therapeutic Drug Monitoring (TDM): Supports the development and validation of assays to monitor drug concentrations in patients for optimal dosing.
• Veterinary and Food Safety Analysis: Used in methods to detect and quantify sulfamethoxazole residues in animal-derived food products.
• Metabolite Identification and Profiling: Aids in the structural confirmation and quantification of acetylated metabolic pathways.
• Method Development and Validation: Serves as a key reference material for establishing robust, compliant analytical protocols (e.g., following FDA/EMA guidelines).

Basic Information

Product Name	n-Acetyl Sulfamethoxazole-D4
CAS No.	1215530-54-3
Molecular Formula	C13H11D4N3O3S
Molecular Weight	293.35 g/mol
Synonyms	N4-Acetylsulfamethoxazole-d4; Sulfamethoxazole N4-Acetyl-d4; 4-Amino-N-(5-methyl-3-isoxazolyl)benzenesulfonamide N4-Acetyl-d4; N1-(5-Methyl-3-isoxazolyl)sulfanilamide 4-Acetamido-d4; Deuterated N-Acetyl Sulfamethoxazole; SMX Acetyl Metabolite-d4; Internal Standard for Sulfamethoxazole LC-MS
EINECS	Contact for details

Quality Control

This deuterated standard is manufactured under strict quality control to ensure isotopic purity and chemical integrity suitable for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR and HPLC-MS. A comprehensive Certificate of Analysis (COA) is provided, detailing isotopic enrichment (typically ≥98 atom % D), chemical purity (≥95%), and chromatographic profile. Our quality system supports compliance with ISO 9001 standards for research chemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This compound is hygroscopic (moisture-sensitive); keep the container tightly sealed in a dry environment to maintain stability and purity. Avoid prolonged exposure to high humidity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time corresponds to reference
Purity (HPLC)	≥ 95%
Isotopic Purity	≥ 98 atom % D
Chemical Purity	≥ 95%
Water Content	≤ 1.0% (KF)
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.