Description

Midazolam 2,5-Dioxide-D6 is a deuterated isotopologue of a key benzodiazepine metabolite, specifically designed for use as an internal standard in advanced analytical applications. This high-purity compound is essential for ensuring accuracy and reliability in quantitative mass spectrometry, eliminating variability and improving data integrity. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and forensic toxicology units for precise drug metabolism and pharmacokinetic (DMPK) studies, bioanalytical method development, and regulatory compliance testing.

Application

• Internal Standard for LC-MS/MS: Critical for the accurate quantification of Midazolam and its metabolites in complex biological matrices like plasma, serum, and urine.
• Drug Metabolism Studies (DMPK): Used in pharmaceutical research to track drug disposition, metabolic pathways, and pharmacokinetic profiles with high precision.
• Forensic and Clinical Toxicology: Enables reliable screening and confirmation of benzodiazepine exposure in forensic investigations and clinical casework.
• Bioanalytical Method Development and Validation: Serves as a stable isotope-labeled reference compound to develop robust, sensitive, and selective analytical methods compliant with FDA/EMA guidelines.
• Pharmacology Research: Supports studies on cytochrome P450 enzyme activity, particularly CYP3A4 phenotyping, due to Midazolam's role as a probe substrate.
• Reference Material for Certified Standards: Used by manufacturers of certified reference materials (CRMs) to produce calibrated standards for quality control laboratories.

Basic Information

Product Name	Midazolam 2,5-Dioxide-D6
CAS No.	1215321-98-4
Molecular Formula	C₁₈H₁₀D₆ClFN₃O₃
Molecular Weight	389.83 g/mol (approximate)
Synonyms	8-Chloro-6-(2-fluorophenyl)-1-methyl-4H-imidazo[1,5-a][1,4]benzodiazepine-2,5(1H,3H)-dione-1,3,4,6,7,8-d6; Midazolam 1,3,4,6,7,8-d6 2,5-Dioxide; Deuterated Midazolam Dioxide; Midazolam-d6 2,5-Dioxide; [1,3,4,6,7,8-d6]-Midazolam-2,5-dione; Stable Isotope Labeled Midazolam Metabolite
EINECS	Contact for details

Quality Control

Every batch of Midazolam 2,5-Dioxide-D6 is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity, verified by advanced spectroscopic and chromatographic techniques including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing batch-specific results for identity, purity (HPLC assay), isotopic enrichment, and residual solvents, ensuring traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.