Description

Hydroxy Metronidazole-D4 is a deuterium-labeled analog of the primary metabolite of the antibiotic metronidazole. This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry-based analyses, ensuring high accuracy and reproducibility. It is a critical tool for researchers and quality control laboratories in the pharmaceutical and contract research organization (CRO) sectors, particularly for pharmacokinetic studies, drug metabolism research, and bioanalytical method development.

Application

• Internal Standard for LC-MS/MS: Primary application as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for bioanalysis.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in studies to track the absorption, distribution, metabolism, and excretion (ADME) of metronidazole and its metabolites.
• Clinical Research: Enables precise measurement of metronidazole and hydroxy-metronidazole levels in biological matrices (plasma, serum, urine) during clinical trials.
• Forensic and Toxicological Analysis: Supports accurate quantification in forensic investigations or toxicology reports where metronidazole exposure is relevant.
• Pharmaceutical Quality Control: Applied in the development and validation of analytical methods for drug substance and product testing.
• Biomedical Research: Facilitates mechanistic studies on the activity and metabolic pathways of nitroimidazole antibiotics.

Basic Information

Product Name	Hydroxy Metronidazole-D4
CAS No.	1215071-08-1
Molecular Formula	C6H5D4N3O4
Molecular Weight	175.17 g/mol (for unlabeled form, C6H9N3O4)
Synonyms	2-Methyl-5-nitroimidazole-1-ethanol-d4, 2-Hydroxymethyl-1-methyl-5-nitroimidazole-d4; 1-(2-Hydroxyethyl)-2-methyl-5-nitroimidazole-d4; Metronidazole Hydroxy Metabolite-d4; MNZ-OH-d4; Hydroxymetronidazole-d4; Deuterated Hydroxy Metronidazole
EINECS	Contact for details

Quality Control

Every batch of Hydroxy Metronidazole-D4 is manufactured and analyzed under strict quality management systems. We provide comprehensive analytical data to support its use in regulated environments, including certificates of analysis (COA) detailing isotopic purity, chemical purity, and absence of interfering impurities. Our quality commitment ensures the material meets the stringent requirements for bioanalytical method validation and research applications. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label or COA. For long-term storage, consider storing desiccated at -20°C. Handle and store in accordance with good laboratory practices.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Purity (D-content)	≥98.0 atom % D
Chemical Purity (NMR)	Conforms
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.