Description

Nicotinic Acid-13C6, D4 n-Hydroxysuccinimide Ester is a high-purity, isotopically labeled derivative of nicotinic acid, specifically designed for advanced analytical and research applications. This compound serves as a critical reagent for the precise quantification and metabolic tracing of nicotinic acid (vitamin B3) and its derivatives in complex biological matrices. It is an essential tool for researchers and analytical scientists in the pharmaceutical, nutritional science, and clinical diagnostics industries who require the highest levels of accuracy and sensitivity in their LC-MS/MS and NMR-based assays.

Application

• Internal Standard for Quantitative Bioanalysis: Primary use as a stable isotope-labeled internal standard (SIL-IS) for the accurate LC-MS/MS quantification of nicotinic acid and its metabolites in plasma, serum, and tissue samples.
• Metabolic Pathway Tracing: Employed in tracer studies to investigate the absorption, distribution, metabolism, and excretion (ADME) of vitamin B3 using 13C and deuterium (2H) labels.
• Pharmaceutical R&D: Critical for the pharmacokinetic (PK) and pharmacodynamic (PD) studies of new drug candidates targeting niacin-related pathways or utilizing nicotinic acid as a precursor.
• Clinical Diagnostics Development: Used in the development and validation of high-sensitivity diagnostic assays for inborn errors of metabolism related to tryptophan-nicotinamide pathways.
• Nutritional Science Research: Enables precise measurement of niacin status, bioavailability from fortified foods, and supplements in human and animal studies.
• Bioconjugation & Probe Synthesis: The active NHS ester moiety allows for efficient and stable conjugation to amines, useful for creating fluorescent or affinity probes for target identification.

Basic Information

Item	Details
Product Name	Nicotinic Acid-13C6, D4 n-Hydroxysuccinimide Ester
CAS No.	1209457-55-5
Molecular Formula	C613C6H4D4N2O4
Molecular Weight	~221.18 g/mol (labeled)
Synonyms	Nicotinic Acid-13C6, D4 NHS Ester; Niacin-13C6, D4 N-Hydroxysuccinimide Ester; 3-Carboxypyridine-13C6, D4 NHS Ester; Vitamin B3-13C6, D4 Active Ester; Pyridine-3-carboxylic Acid-13C6, D4 Succinimidyl Ester; [13C6, D4]-Nicotinic Acid NHS Ester; Stable Isotope Labeled Nicotinic Acid NHS Ester; SIL-Nicotinic Acid NHS Ester
EINECS	Contact for details

Quality Control

Every batch of Nicotinic Acid-13C6, D4 n-Hydroxysuccinimide Ester is manufactured and analyzed under strict quality systems to ensure the highest isotopic purity and chemical integrity required for sensitive mass spectrometry applications. Our products undergo rigorous quality testing, including NMR and LC-MS, to verify isotopic enrichment and chemical purity. Certificates of Analysis (COA) are provided with each shipment, detailing batch-specific results for isotopic purity, chemical purity, and solvent content. We support compliance with GMP/GLP guidelines for research use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This compound is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider storing under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Chemical Purity)
Isotopic Purity	≥ 99 atom % 13C; ≥ 98 atom % D
Water Content (KF)	≤ 1.0 %
Solvent Content (Residual Solvents)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.