Description

6-Carboxyfluorescein-Azide is a high-purity, functionalized fluorescent dye derivative designed for advanced bioconjugation and labeling applications. This compound is critical for researchers and developers requiring precise, covalent attachment of fluorescent tags to biomolecules via click chemistry. It is primarily utilized in the life sciences, diagnostics, and pharmaceutical development sectors for applications such as bioimaging, flow cytometry, and assay development.

Application

• Bioorthogonal Labeling: Covalent attachment to alkyne-modified biomolecules (proteins, antibodies, nucleic acids) via copper-catalyzed azide-alkyne cycloaddition (CuAAC) for fluorescence tagging.
• Live-Cell Imaging Probes: Synthesis of targeted fluorescent probes for real-time visualization of cellular processes and structures.
• Flow Cytometry & FACS: Conjugation to antibodies or other targeting ligands for high-sensitivity cell sorting and population analysis.
• Diagnostic Assay Development: Used as a key component in the development of fluorescent immunoassays (e.g., ELISA) and lateral flow tests.
• Polymer & Materials Science: Fluorescent labeling of polymers, nanoparticles, and surfaces to study material interactions and distribution.
• Protein & Peptide Tracking: Monitoring protein-protein interactions, localization, and turnover in biochemical studies.

Basic Information

Product Name	6-Carboxyfluorescein-Azide
CAS No.	1204815-86-0
Molecular Formula	C22H13N3O6
Molecular Weight	415.36 g/mol
Synonyms	6-FAM Azide; 5(6)-Carboxyfluorescein Azide; 6-Carboxyfluorescein N3; 6-FAM-N3; Fluorescein-6-Carboxamido-azide; 6-CF Azide; Azido-6-carboxyfluorescein; 6-Carboxyfluorescein, 6-isomer, azide derivative
EINECS	Contact for details

Quality Control

Our 6-Carboxyfluorescein-Azide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and spectroscopic methods for structural confirmation, to ensure it meets the high standards required for sensitive research and development applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥95%
Absorption (λmax)	~492 nm
Emission (λmax)	~517 nm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.