Description

5-{[({3-[(2,5-Dioxo-1-Pyrrolidinyl)Oxy]-3-Oxopropyl}Sulfanyl)Acetyl]Amino}-2-(6-Hydroxy-3-Oxo-3H-Xanthen-9-Yl)Benzoic Acid is a sophisticated heterobifunctional crosslinking reagent that combines a maleimide-activated ester with a fluorescent xanthene (fluorescein) core. This compound is engineered for the site-specific conjugation of biomolecules, enabling the creation of stable, fluorescently labeled probes for advanced research and diagnostics. It is an essential tool for scientists in bioconjugation, assay development, and cellular imaging, offering precise control over labeling chemistry.

Application

• Fluorescent Probe Synthesis: Conjugation of peptides, proteins, and antibodies with fluorescein for fluorescence microscopy (FLIM, FRET) and flow cytometry.
• Diagnostic Assay Development: Labeling of detection antibodies or antigens for use in ELISA, lateral flow assays, and other immunoassays.
• Bioconjugation & Linker Chemistry: As a heterobifunctional crosslinker for creating stable thioether bonds between sulfhydryl groups and primary amines.
• Cellular & Molecular Imaging: Preparation of fluorescently tagged small molecules or nanoparticles for tracking cellular uptake and localization.
• Drug Delivery Research: Conjugation of fluorescent markers to drug carriers or targeting ligands to study pharmacokinetics and biodistribution.
• Biomaterial Functionalization: Introducing fluorescent labels onto surfaces or polymers for biosensor and tissue engineering applications.

Basic Information

Item	Details
Product Name	5-{[({3-[(2,5-Dioxo-1-Pyrrolidinyl)Oxy]-3-Oxopropyl}Sulfanyl)Acetyl]Amino}-2-(6-Hydroxy-3-Oxo-3H-Xanthen-9-Yl)Benzoic Acid
CAS No.	1196157-68-2
Molecular Formula	C29H20N2O10S
Molecular Weight	588.54 g/mol
Synonyms	5-((3-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)propanoylthio)acetamido)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid; Fluorescein-5-maleimide; 5-FAM Maleimide; Maleimide-activated Fluorescein; SMCC-Fluorescein derivative; Fluorescein based heterobifunctional crosslinker; Fluorescent Maleimide reagent
EINECS	Contact for details

Quality Control

Every batch of this high-purity biochemical reagent is produced under strict quality management and undergoes comprehensive analytical characterization. We ensure compliance with rigorous internal specifications for critical parameters including identity, purity, and functional activity. A detailed Certificate of Analysis (COA) with supporting HPLC, NMR, and MS data is provided to guarantee batch-to-batch consistency and performance for your sensitive applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Functional Activity	Conjugation efficiency confirmed by model reaction

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.